Purification of erythropoietin-responsive cells by immune hemolysis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 6 ·1972-06-00 ·Pages 1337-41

Cantor LN, Morris AJ, Marks PA, Rifkind RA

Abstract

Immature erythroid cell precursors from 12- and 13-day fetal mouse livers were concentrated by differential antibody-mediated hemolysis. The effect of erythropoietin on these purified erythroid cell precursors was studied in vitro. Addition of erythropoietin stimulates erythroid cells to proliferate, and to differentiate to cells that actively synthesize hemoglobin. This erythropoietin-induced erythropoiesis is sustained in culture for at least 48 hr.

MeSH Terms

Animals Antigen-Antibody Reactions Cell Differentiation/drug effects Complement System Proteins Erythrocytes/drug effects,immunology Erythropoiesis/drug effects Erythropoietin/pharmacology Female Fetus/immunology Gestational Age Guinea Pigs Hemoglobins/biosynthesis Hemolysis Humans Immune Sera Immunization Liver/embryology,immunology Mice Mice, Inbred Strains Rabbits/immunology Species Specificity Stimulation, Chemical

Chemicals

Hemoglobins Immune Sera Erythropoietin Complement System Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Cantor L N

Morris A J

Marks P A

Rifkind R A

References (13)

13 references, click to expand

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Differential immune lysis of erythroblasts.
Nature. 1969 Mar 29;221(5187):1261-2 PMID: 5773847
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Trans Assoc Am Physicians. 1969;82:380-7 PMID: 4192750
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J Cell Biol. 1971 Dec;51(3):585-95 PMID: 5128349
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Science. 1972 Mar 3;175(4025):955-61 PMID: 5061867
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THE DISTRIBUTION OF BUOYANT DENSITY OF HUMAN ERYTHROCYTES IN BOVINE ALBUMIN SOLUTIONS.
Proc Natl Acad Sci U S A. 1964 Mar;51:520-8 PMID: 14171467
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.6.1337

Published

1972-06-00

Pages

1337-41

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426696

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.6.1337 PMC Full Text View journal details

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Purification of erythropoietin-responsive cells by immune hemolysis.

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