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PMID: 4504354 Published · ppublish English Journal Article

Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 6 ·1972-06-00 ·Pages 1425-8

Jacobs-Lorena M, Baglioni C

Abstract

10S RNA has been isolated from the postribosomal supernatant of rabbit reticulocyte lysate. This RNA is associated with protein in a ribonucleoprotein particle that sediments at approximately 20S. The 10S RNA extracted from this particle codes predominantly for alpha globin chains when it is translated by a mouse ascites cell-free extract. The product of the cell-free synthesis has been characterized by column chromatography and electrophoresis of the tryptic peptides. About 20% of the total alpha chain mRNA is present in the postribosomal supernatant, whereas beta chain mRNA is almost exclusively associated with polyribosomes.

MeSH Terms
Animals Carcinoma, Krebs 2/metabolism Cell Fractionation Cell-Free System Centrifugation, Density Gradient Chromatography Electrophoresis Genetic Code Globins/analysis Mice Neoplasm Proteins/biosynthesis Peptide Chain Initiation, Translational/drug effects RNA, Messenger/isolation & purification,pharmacology Rabbits Reticulocytes/analysis Trypsin
Chemicals
Neoplasm Proteins RNA, Messenger Globins Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jacobs-Lorena M
Baglioni C
References (15)
15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.6.1425
Published
1972-06-00
Pages
1425-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426717
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.6.1425 PMC Full Text View journal details
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