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PMID: 4504367 Published · ppublish English Journal Article

A novel DNA polymerase activity found in association with intracisternal A-type particles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 6 ·1972-06-00 ·Pages 1531-6

Wilson SH, Kuff EL

Abstract

A DNA polymerase activity that promotes the synthesis of poly(dT) has been found in association with intracisternal A-type particles isolated from several mouse tumors. The poly(dT) synthesis activity requires a DNA or RNA primer, is optimal at high salt concentration, prefers magnesium over manganese, and is stimulated by poly(rA). No significant incorporation of dAMP, dGMP, or dCMP was detected in the presence of several RNA and DNA template-primers. The enzyme activity differs in several of its properties from the poly(rA)-directed DNA polymerase activity associated with Rauscher murine leukemia virus.

MeSH Terms
Adenine Nucleotides/pharmacology Animals Cytoplasm/enzymology,metabolism DNA Nucleotidyltransferases/metabolism Enzyme Activation/drug effects Inclusion Bodies, Viral Magnesium/pharmacology Manganese/pharmacology Mice Mice, Inbred Strains Multiple Myeloma/enzymology,metabolism Neoplasms, Experimental/enzymology,metabolism Polynucleotides/biosynthesis Potassium Chloride/pharmacology Rauscher Virus/enzymology Templates, Genetic Thymine Nucleotides/biosynthesis Tritium
Chemicals
Adenine Nucleotides Polynucleotides Thymine Nucleotides Tritium Manganese Potassium Chloride DNA Nucleotidyltransferases Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wilson S H
Kuff E L
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.6.1531
Published
1972-06-00
Pages
1531-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426742
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.6.1531 PMC Full Text View journal details
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