Assays for porphyrins, delta-aminolevulinic-acid dehydratase, and porphyrinogen synthetase in microliter samples of whole blood: applications to metabolic defects involving the heme pathway.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 9 ·1972-09-00 ·Pages 2381-5

Granick S, Sassa S, Granick JL, Levere RD, Kappas A

Abstract

Three micromethods are described for the assay of enzymes or products of the heme biosynthetic pathway in blood. A fluorometric method for the assay of protoporphyrin may be applied to the rapid screening of children for chronic lead poisoning. A colorimetric assay for delta-aminolevulinic-acid dehydratase may be applied to the detection of acute and chronic lead poisoning. A fluorometric assay for porphyrin formation from prophobilinogen is also described.

MeSH Terms

Acute Disease Anemia/blood,diagnosis,enzymology Anemia, Hypochromic/blood,enzymology Chronic Disease Colorimetry Erythrocytes/analysis Fluorometry Humans Hydro-Lyases/blood Lead Poisoning/blood,diagnosis,enzymology Levulinic Acids Ligases/blood Methods Porphyrias/blood,diagnosis,enzymology Porphyrins/blood Spectrometry, Fluorescence/instrumentation

Chemicals

Levulinic Acids Porphyrins Hydro-Lyases Ligases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Granick S

Sassa S

Granick J L

Levere R D

Kappas A

References (8)

8 references, click to expand

A micro-sampling method for the rapid determination of lead in blood by atomic-absorption spectrophotometry.
Analyst. 1970 May;95(130):431-8 PMID: 5419713
Enzymatic studies on erythroid differentiation and proliferation.
Ann N Y Acad Sci. 1968 Mar 29;149(1):224-8 PMID: 5240711
The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine.
J Biol Chem. 1956 Mar;219(1):435-46 PMID: 13295297
Intermittent acute porphyria--demonstration of a genetic defect in porphobilinogen metabolism.
N Engl J Med. 1972 Jun 15;286(24):1277-82 PMID: 5024458
Delta-aminolevulinic acid dehydratase activity in circulating blood cells. A sensitive laboratory test for the detection of childhood lead poisoning.
N Engl J Med. 1971 Mar 18;284(11):565-9 PMID: 5100889
Porphyria.
Adv Intern Med. 1954;6:235-99 PMID: 13171164
DISTURBANCES IN THE BIOSYNTHESIS OF HEME IN LEAD INTOXICATION.
J Pediatr. 1964 Feb;64:174-87 PMID: 14119517
Spectral-absorption coefficients of some porphyrins in the Soret-band region.
Biochem J. 1960 Jun;75(3):620-3 PMID: 16748818

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.9.2381

Published

1972-09-00

Pages

2381-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426945

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.9.2381 PMC Full Text View journal details

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Assays for porphyrins, delta-aminolevulinic-acid dehydratase, and porphyrinogen synthetase in microliter samples of whole blood: applications to metabolic defects involving the heme pathway.

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