Regeneration of cation-transport capacity in HeLa cell membranes after specific blockade by ouabain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 9 ·1972-09-00 ·Pages 2627-31

Vaughan GL, Cook JS

Abstract

The cardiac glycoside, ouabain, inhibits alkali-cation transport in HeLa cells. It binds to 0.75 x 10(6) sites per cell, and the half-time for its dissociation is 16 hr. After partial blockade by ouabain, the cell generates new ouabain-binding sites, with total restoration of transport in 10% of a cell cycle( approximately 3 hr). This recovery requires protein synthesis and appears to be a response to altered cell-electrolyte content, since growth of cells in media with low K(+) concentration enhances the titer of the transport enzyme in a fashion similar to the effect of ouabain. Totally blocked cells do not recover.

MeSH Terms

Animals Binding Sites Biological Transport/drug effects Cell Line Cells, Cultured Cycloheximide/pharmacology HeLa Cells/drug effects,growth & development,metabolism Kinetics Leucine/metabolism Mice Neoplasm Proteins/biosynthesis Ouabain/metabolism,pharmacology Potassium/pharmacology Radioisotopes Rubidium/metabolism Tritium

Chemicals

Neoplasm Proteins Radioisotopes Tritium Ouabain Cycloheximide Leucine Rubidium Potassium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Vaughan G L

Cook J S

References (13)

13 references, click to expand

Membranes of animal cells. II. The metabolism and turnover of the surface membrane.
J Cell Biol. 1968 Jun;37(3):729-46 PMID: 11905204
Monovalent cation requirement for the aminoacyl transfer reaction in protein synthesis.
Nature. 1966 Sep 17;211(5055):1302-3 PMID: 5339002
Cation metabolism in relation to cell size in synchronously grown tissue culture cell.
J Gen Physiol. 1967 Mar;50(4):917-32 PMID: 6034509
Virus-receptor interaction in an adenovirus system.
J Virol. 1968 Oct;2(10):1064-75 PMID: 5723709
[On the influence of radiation-induced electropyte displacements on metabolism. I. Studies on acetyl-CoA synthetase in yeast].
Strahlentherapie. 1968 Mar;135(3):354-63 PMID: 5724683
On the number of sodium pumping sites in cell membranes.
Biochim Biophys Acta. 1969;183(3):646-9 PMID: 5822834
Potassium ions and the binding of cardiac glycosides to mammalian cells.
Nature. 1970 May 9;226(5245):521-3 PMID: 5442345
Uptake of (3H)ouabain and Na pump turnover rates in monolayer cultures of Girardi heart cells.
J Physiol. 1971 Mar;213(2):57P-58P PMID: 5574859
Regeneration of transplantation antigens on mouse cells.
Transplant Proc. 1971 Mar;3(1):180-2 PMID: 4106304
Cholesterol metabolism in the macrophage. II. Alteration of subcellular exchangeable cholesterol compartments and exchange in other cell types.
J Exp Med. 1971 Dec 1;134(6):1570-90 PMID: 4942408
Regeneration of the surface glycoproteins of a transplantable mouse tumor cell after treatment with neuraminidase.
Proc Natl Acad Sci U S A. 1972 Apr;69(4):942-5 PMID: 4502944
THE ACTION OF CARDIAC GLYCOSIDES ON ION MOVEMENTS.
Pharmacol Rev. 1964 Dec;16:381-407 PMID: 14240177
Potassium movements in washed erythrocytes.
J Physiol. 1955 Sep 28;129(3):464-75 PMID: 13264110

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.9.2627

Published

1972-09-00

Pages

2627-31

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427003

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.9.2627 PMC Full Text View journal details

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Regeneration of cation-transport capacity in HeLa cell membranes after specific blockade by ouabain.

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