The respective roles of the protein kinase and pppA2' p5' A2' p5 A-activated endonuclease in the inhibition of protein synthesis by double stranded RNA in rabbit reticulocyte lysates.

Nucleic acids research ·Vol. 6 ·No. 4 ·1979-04-00 ·Pages 1335-50

Williams BR, Gilbert CS, Kerr IM

Abstract

Double-stranded RNA (dsRNA) inhibits protein synthesis in rabbit reticulocyte lysates by activating the synthesis of the endonuclease effector pppA2' p5' A2' p5' A(2-5A) and a protein kinase which phosphorylates the protein synthesis initiation factor eIF-2. Under certain assay conditions, high concentrations of dsRNA are without inhibitory effect in many lysates (high dsRNA "reversible" lysates). In these lysates natural dsRNA at low concentrations stimulated protein kinase activity to a greater extent than did the synthetic dsRNA poly rI.rC. Synthesis of 2--5A was greater when poly rI.rC was used. However, a number of factors, including the salt concentration and messenger RNA used, combine to determine the overall effect of dsRNA on protein synthesis under any given set of experimental conditions.

MeSH Terms

Adenine Nucleotides/pharmacology Animals Endonucleases/metabolism Kinetics Oligonucleotides/pharmacology Oligoribonucleotides/pharmacology Potassium Chloride/pharmacology Protein Biosynthesis/drug effects Protein Kinases/metabolism RNA/metabolism Rabbits Reticulocytes/metabolism Ribonucleases/metabolism

Chemicals

Adenine Nucleotides Oligonucleotides Oligoribonucleotides RNA Potassium Chloride Protein Kinases Endonucleases Ribonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Williams B R

Gilbert C S

Kerr I M

References (18)

18 references, click to expand

The (2'-5') oligoadenylate (pppA2'-5'A2'-5'A) synthetase and protein kinase(s) from interferon-treated cells.
Eur J Biochem. 1979 Feb 1;93(3):515-26 PMID: 217683
The effect of salt concentration on the inhibition of protein synthesis by double-stranded RNA.
Eur J Biochem. 1978 Dec 1;92(1):155-63 PMID: 729587
Synthesis and breakdown of pppA2'p5'A2'p5'A and transient inhibiton of protein synthesis in extracts from interferon-treated and control cells.
Eur J Biochem. 1978 Dec;92(2):455-62 PMID: 216547
Synthesis of an oligonucleotide inhibitor of protein synthesis in rabbit reticulocyte lysates analogous to that formed in extracts from interferon-treated cells.
Eur J Biochem. 1978 Mar;84(1):149-59 PMID: 206430
Inhibition of cell-free protein synthesis by pppA2'p5'A2'p5'A: a novel oligonucleotide synthesized by interferon-treated L cell extracts.
Cell. 1978 Mar;13(3):565-72 PMID: 657268
Interferon action: isolation of nuclease F, a translation inhibitor activated by interferon-induced (2'-5') oligo-isoadenylate.
FEBS Lett. 1978 Nov 15;95(2):257-64 PMID: 720619
pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells.
Proc Natl Acad Sci U S A. 1978 Jan;75(1):256-60 PMID: 272640
Coupled transcription and translation in mammalian and avian cell-free systems.
Virology. 1978 Feb;84(2):479-95 PMID: 203097
Interferon action may be mediated by activation of a nuclease by pppA2'p5'A2'p5'A.
Nature. 1978 Jun 22;273(5664):684-7 PMID: 208001
Effect of interferon pretreatment on coupled transcription and translation in cell-free extracts of primary chick embryo cells.
Virology. 1978 Feb;84(2):496-508 PMID: 203098
Interferon action: two distinct pathways for inhibition of protein synthesis by double-stranded RNA.
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5893-7 PMID: 282611
Phosphorylation of initiation factor elF-2 and the control of reticulocyte protein synthesis.
Cell. 1977 May;11(1):187-200 PMID: 559547
Inhibition of protein synthesis by 2'-5' linked adenine oligonucleotides in intact cells.
Nature. 1978 Nov 2;276(5683):88-90 PMID: 740027
Differential effects of various double-stranded RNAs on protein synthesis in rabbit reticulocyte lysates.
Biochemistry. 1978 Jan 10;17(1):88-94 PMID: 618549
An efficient mRNA-dependent translation system from reticulocyte lysates.
Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
Control of globin synthesis: the role of heme.
J Mol Biol. 1972 May 28;66(3):471-81 PMID: 5037023
Met-tRNAfMet binding to 40S ribosomal subunits: a site for the regulation of initiation of protein synthesis by hemin.
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2946-50 PMID: 4528641
Increased sensitivity of cell-free protein synthesis to double-stranded RNA after interferon treatment.
Nature. 1974 Jul 5;250(461):57-9 PMID: 4366491

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.4.1335

Published

1979-04-00

Pages

1335-50

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327776

Subset

External Links

PubMed source DOI: 10.1093/nar/6.4.1335 PMC Full Text View journal details

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The respective roles of the protein kinase and pppA2' p5' A2' p5 A-activated endonuclease in the inhibition of protein synthesis by double stranded RNA in rabbit reticulocyte lysates.

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