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PMID: 450716 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Antibiotic induced electrophoretic mobility shifts of DNA restriction fragments.

Nucleic acids research ·Vol. 6 ·No. 5 ·1979-00-00 ·Pages 1869-79

Loucks E, Chaconas G, Blakesley RW, Wells RD, van de Sande JH

Abstract

Several antibiotics, netropsin, distamycin A, actinomycin D, Hoechst 33258 and olivomycin, which demonstrate base specificity in their DNA binding properties have been found to alter the electrophoretic mobility of DNA restriction fragments in native polyacrylamide gels. The antibiotics mostly reduced the migration of larger DNA fragments, but netropsin and Hoechst 33258 were observed to increase the migration rate of several DNA fragments of intermediate size. DNA fragments of similar molecular weight which comigrate as a single gel band can at times be separated as the result of differential mobility shifts promoted by antibiotic DNA complex formations.

MeSH Terms
Anti-Bacterial Agents Chemical Phenomena Chemistry DNA Restriction Enzymes DNA, Viral Dactinomycin Distamycins Electrophoresis, Polyacrylamide Gel Ethidium Molecular Weight Netropsin Olivomycins
Chemicals
Anti-Bacterial Agents DNA, Viral Distamycins Dactinomycin Olivomycins Netropsin DNA Restriction Enzymes Ethidium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Loucks E
Chaconas G
Blakesley R W
Wells R D
van de Sande J H
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.5.1869
Published
1979-00-00
Pages
1869-79
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327817
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.5.1869 PMC Full Text View journal details
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