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PMID: 450721 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Photochemical cross-linking of secondary structure in HeLa cell heterogeneous nuclear RNA in situ 1.

Nucleic acids research ·Vol. 6 ·No. 5 ·1979-00-00 ·Pages 1993-2001

Calvet JP, Pederson T

Abstract

The psoralen derivative 4'-hydroxymethyl-4, 5', 8-trimethylpsoralen (hydroxymethyltrioxsalen) has been used in experiments with isolated HeLa cell nuclei to photochemically cross-link double helical regions in heterogeneous nuclear RNA in situ. Although there are other self-complementary sequences in hnRNA that can form base-paired structures upon phenol deproteinization of annealing, the present in situ cross-linking results demonstrate that some double-stranded regions are an authentic component of native hnRNA structure. Moreover, these special regions of secondary structure are apparently highly accessible to chemical probes within the intact cell nucleus, despite the fact that hnRNA possesses a ribonucleoprotein organization.

MeSH Terms
Base Sequence Cell Nucleus/metabolism HeLa Cells/drug effects,metabolism,radiation effects Nucleic Acid Conformation Nucleic Acid Renaturation RNA, Heterogeneous Nuclear/metabolism,radiation effects Trioxsalen/analogs & derivatives,pharmacology Ultraviolet Rays
Chemicals
RNA, Heterogeneous Nuclear Trioxsalen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Calvet J P
Pederson T
References (22)
22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.5.1993
Published
1979-00-00
Pages
1993-2001
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327826
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.5.1993 PMC Full Text View journal details
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