Fusion of mammalian somatic cells by microsurgery.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 10 ·1972-10-00 ·Pages 2959-62

Diacumakos EG, Tatum EL

Abstract

Mammalian somatic-cell hybrids can be formed in a direct and precise manner by microsurgery. Fusion of cells brought about in this way permits selection of specific, identifiable cells of the same or different types or species and their fusion at telophase.

MeSH Terms

Animals Cell Division Cell Fusion Cell Line Clone Cells Fetus HeLa Cells Humans Hybrid Cells Hybridization, Genetic L Cells Lesch-Nyhan Syndrome Mice Microsurgery

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Diacumakos E G

Tatum E L

References (8)

8 references, click to expand

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Exp Cell Res. 1968 Sep;52(1):34-42 PMID: 4300100
A microsurgical methodology for human cells in vitro: evolution and applications.
Proc Natl Acad Sci U S A. 1970 Apr;65(4):911-8 PMID: 5266161
Microsurgical studies on human cells and cloning of HeLa cells.
Nature. 1971 Jul 2;232(5305):28-32 PMID: 4933169
Chromosome displacement in and extraction from human cells at different mitotic stages.
Nature. 1971 Jul 2;232(5305):33-6 PMID: 4933170
Induction of homokaryocyte, heterokaryocyte and hybrid formation by lysolecithin.
Exp Cell Res. 1971 Aug;67(2):427-35 PMID: 4328995
Cell fusion without viruses.
Biochem J. 1971 Oct;124(5):46P-47P PMID: 5002195
Effect of environmental pH on the efficiency of cellular hybridization.
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1953-6 PMID: 4340167
Poliovirus replication and cytopathogenicity in monolayer hamster cell cultures fused with beta propiolactone-inactivated Sendai virus.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.10.2959

Published

1972-10-00

Pages

2959-62

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389683

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.10.2959 PMC Full Text View journal details

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Fusion of mammalian somatic cells by microsurgery.

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