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PMID: 4507620 Published · ppublish English Journal Article

Nucleotide sequence at the binding site for coat protein on RNA of bacteriophage R17.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 10 ·1972-10-00 ·Pages 3033-7

Bernardi A, Spahr PF

Abstract

The binding of a few molecules [1-6] of RNA bacteriophage coat protein to 1 molecule of RNA represses in vitro translation of the RNA synthetase cistron. Digestion of the complex, R17 coat protein-R17 RNA, by T1 RNase yields an RNA fragment bound to the coat protein. The nucleotide sequence of this fragment (59 residues) reveals that it contains the punctuation signal between the coat protein and RNA synthetase cistrons, suggesting that this is the site on the RNA where the coat protein acts as a translational repressor.

MeSH Terms
Acid Phosphatase Bacteriophages/analysis,metabolism Base Sequence Binding Sites Centrifugation, Density Gradient Coliphages/enzymology Electrophoresis, Polyacrylamide Gel Genes, Regulator Oligonucleotides/analysis Pancreas/enzymology Phosphorus Isotopes Protein Binding Protein Biosynthesis RNA, Viral/analysis,metabolism Ribonucleases Spleen/enzymology Tritium Viral Proteins/analysis,metabolism
Chemicals
Oligonucleotides Phosphorus Isotopes RNA, Viral Viral Proteins Tritium Ribonucleases Acid Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bernardi A
Spahr P F
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.10.3033
Published
1972-10-00
Pages
3033-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389701
Subset
IM
Databases
GENBANK
J02488
External Links
PubMed source DOI: 10.1073/pnas.69.10.3033 PMC Full Text View journal details
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