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PMID: 4508312 Published · ppublish English Journal Article

Acetylcholine receptors of muscle grown in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 11 ·1972-11-00 ·Pages 3180-4

Vogel Z, Sytkowski AJ, Nirenberg MW

Abstract

[(125)I]Monoiodo- and [(125)I]diiodo-alpha-bungarotoxin were synthesized and shown to bind specifically to the acetylcholine receptor of cultured embryonic chick- and rat-muscle cells. The pharmacologic properties of the receptor of cultured embryonic chick muscle resembled those of the nicotinic acetylcholine receptor of adult vertebrate muscle. Autoradiography of muscle cells labeled with toxin showed that acetylcholine receptors were distributed over the entire cell surface. In addition, discrete areas with a high receptor concentration were found.

MeSH Terms
Acetylcholine/metabolism Animals Atropine/pharmacology Autoradiography Carbachol/pharmacology Chick Embryo Culture Techniques Decamethonium Compounds/pharmacology Gallamine Triethiodide/pharmacology Hexamethonium Compounds/pharmacology Iodine Isotopes Iodoproteins/metabolism Kinetics Muscles/metabolism Nicotine/pharmacology Physostigmine/pharmacology Pilocarpine/pharmacology Piperazines/pharmacology Protein Binding/drug effects Rats Receptors, Cholinergic Receptors, Drug Tubocurarine/pharmacology Venoms/metabolism
Chemicals
Decamethonium Compounds Hexamethonium Compounds Iodine Isotopes Iodoproteins Piperazines Receptors, Cholinergic Receptors, Drug Venoms Pilocarpine Nicotine Atropine Carbachol Physostigmine Acetylcholine Gallamine Triethiodide Tubocurarine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vogel Z
Sytkowski A J
Nirenberg M W
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.11.3180
Published
1972-11-00
Pages
3180-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389730
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.11.3180 PMC Full Text View journal details
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