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PMID: 4508327 Published · ppublish English Journal Article

Nonhistone chromosomal proteins in synchronized HeLa cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 11 ·1972-11-00 ·Pages 3345-9

Bhorjee JS, Pederson T

Abstract

Chromatin was isolated from synchronized HeLa cells at different stages of the cell division cycle and fractionated into DNA, histones, and nonhistone proteins. Electrophoresis of the nonhistone proteins in sodium dodecyl sulfate-polyacrylamide gels revealed a highly reproducible pattern of 22 bands, having estimated molecular weights of 15,000-180,000, with 85% (by mass) over 40,000. The amounts of some nonhistone proteins varied during the cell cycle by as much as 50%, while others remained at a constant level. One group of nonhistone proteins (molecular weight 75,000) was greatly reduced just before the start of DNA replication (S-phase), then returned to normal levels in the mid-S phase. These results are discussed with regard to the possible role of nonhistone proteins in regulating chromosome structure and function.

MeSH Terms
Carbon Isotopes Cell Division Cell Fractionation Chromatin/analysis,isolation & purification DNA, Neoplasm/analysis,biosynthesis Electrophoresis, Disc Electrophoresis, Polyacrylamide Gel HeLa Cells/analysis,cytology,metabolism Histones/analysis Kinetics Leucine/metabolism Mitosis Molecular Weight Nucleoproteins/analysis Sodium Dodecyl Sulfate Thymidine/metabolism Tritium
Chemicals
Carbon Isotopes Chromatin DNA, Neoplasm Histones Nucleoproteins Tritium Sodium Dodecyl Sulfate Leucine Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bhorjee J S
Pederson T
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45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.11.3345
Published
1972-11-00
Pages
3345-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389768
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.11.3345 PMC Full Text View journal details
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