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PMID: 4509331 Published · ppublish English Journal Article

Occurrence of uridylate-rich oligonucleotide regions in heterogeneous nuclear RNA of HeLa cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 12 ·1972-12-00 ·Pages 3684-8

Molloy GR, Thomas WL, Darnell JE

Abstract

Heterogeneous nuclear RNA molecules from HeLa cells contain a specific segment of about 30 nucleotides length that is largely (about 80%) uridylic acid. This oligo(U) segment is located predominantly in the larger (70S-90S) heterogeneous nuclear RNA molecules, and is essentially absent in messenger RNA and 45S ribosomal precursor RNA molecules. The oligo(U) hybridizes rapidly to cellular DNA, suggesting that it is transcribed from the repeated regions of the DNA.

MeSH Terms
Alkaline Phosphatase/metabolism Centrifugation, Density Gradient DNA/metabolism Electrophoresis, Polyacrylamide Gel HeLa Cells/analysis Humans Molecular Weight Nucleic Acid Hybridization Oligonucleotides/analysis,metabolism Phosphorus Isotopes Polynucleotides/analysis RNA/analysis,metabolism RNA, Messenger/analysis,isolation & purification Tritium Uracil Nucleotides/analysis
Chemicals
Oligonucleotides Phosphorus Isotopes Polynucleotides RNA, Messenger Uracil Nucleotides Tritium RNA DNA Alkaline Phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Molloy G R
Thomas W L
Darnell J E
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.12.3684
Published
1972-12-00
Pages
3684-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389849
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.12.3684 PMC Full Text View journal details
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