Bromodeoxyuridine dependence--a new mutation in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 1 ·1973-01-00 ·Pages 138-42

Davidson RL, Bick MD

Abstract

When cells of a Syrian hamster melanoma were grown in increasing concentrations of bromodeoxyuridine (BrdU), lines of mutant cells able to grow well at high concentrations of BrdU were isolated. The mutant cells were characterized by BrdU dependence. In the absence of BrdU, the cells grew very poorly. The requirement for BrdU was specific for both the bromine and the deoxy sugar. The mutant cells incorporated BrdU into the DNA, replacing about 50% of the thymine residues with bromouracil. The reason for the BrdU dependence is not known.

MeSH Terms

Aminopterin/pharmacology Animals Bromodeoxyuridine/metabolism Cell Division Cell Line Centrifugation, Density Gradient Clone Cells Cricetinae Culture Media DNA, Neoplasm/analysis,biosynthesis,radiation effects Hypoxanthines/pharmacology Melanoma/metabolism Molecular Weight Mutation Neoplasms, Experimental/metabolism Phosphates/metabolism Phosphorus Isotopes Radiation Effects Thymidine/metabolism Tritium Ultraviolet Rays

Chemicals

Culture Media DNA, Neoplasm Hypoxanthines Phosphates Phosphorus Isotopes Tritium Bromodeoxyuridine Aminopterin Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Davidson R L

Bick M D

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.1.138

Published

1973-01-00

Pages

138-42

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433201

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.1.138 PMC Full Text View journal details

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Bromodeoxyuridine dependence--a new mutation in mammalian cells.

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