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PMID: 4514321 Published · ppublish English Journal Article

Biosynthesis of reticulocyte membrane proteins by membrane-free polyribosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 5 ·1973-05-00 ·Pages 1526-30

Lodish HF

Abstract

Intact rabbit reticulocyte cells synthesize two predominant species of membrane polypeptides; at least 10 reticulocyte membrane polypeptide species are not produced by the cells. Cell-free extracts of reticulocytes, free of any membranes or membrane-bound polyribosomes, synthesize large amounts of these two membrane polypeptides; one of these polypeptides appears to be modified, probably by loss of 20-40 amino acids, after it is incorporated into the membrane. Deprivation of hemin results in inhibition of synthesis by lysates of membrane proteins, globin, and all other cytoplasmic proteins.

MeSH Terms
Acetates Anemia/chemically induced Animals Autoradiography Blood Proteins/biosynthesis Cell Membrane/metabolism Cell-Free System Electrophoresis, Polyacrylamide Gel Heme/metabolism Hydrogen-Ion Concentration Iontophoresis Methionine/metabolism Methods Molecular Weight Peptides/blood,isolation & purification Polyribosomes/metabolism Rabbits Reticulocytes/metabolism Sodium Dodecyl Sulfate Sulfur Isotopes Trypsin/pharmacology
Chemicals
Acetates Blood Proteins Peptides Sulfur Isotopes Sodium Dodecyl Sulfate Heme Methionine Trypsin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lodish H F
References (35)
35 references, click to expand
  1. Biogenesis of endoplasmic reticulum membranes. I. Structural and chemical differentiation in developing rat hepatocyte.
    J Cell Biol. 1966 Jul;30(1):73-96 PMID: 5966178
  2. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  3. Disposition of the major proteins in the isolated erythrocyte membrane. Proteolytic dissection.
    Biochemistry. 1971 Jun 22;10(13):2617-24 PMID: 4104271
  4. Fractionation of the protein components of human erythrocyte membranes.
    J Biol Chem. 1969 Oct 10;244(19):5118-24 PMID: 5344126
  5. Effects of hemin and other porphyrins on protein synthesis in a reticulocyte lysate cell-free system.
    J Mol Biol. 1969 Jun 14;42(2):247-58 PMID: 5803298
  6. Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver.
    J Biol Chem. 1969 Aug 25;244(16):4308-15 PMID: 5806579
  7. Position of glycoprotein polypeptide chain in the human erythrocyte membrane.
    FEBS Lett. 1971 Oct 15;18(1):95-97 PMID: 11946092
  8. Secondary structure of bacteriophage f2 ribonucleic acid and the initiation of in vitro protein biosynthesis.
    J Mol Biol. 1970 Jun 28;50(3):689-702 PMID: 4920497
  9. Biosynthesis of nonglobin protein by membrane-bound ribosomes in reticulocytes.
    J Biol Chem. 1970 Oct 10;245(19):4907-12 PMID: 5506264
  10. Polypeptide chains from human red blood cell membranes.
    J Biol Chem. 1971 Jul 25;246(14):4485-8 PMID: 4999065
  11. On the attachment of ribosomes to microsomal membranes.
    J Mol Biol. 1966 Aug;19(2):503-24 PMID: 4961331
  12. Sulfanilic acid diazonium salt: a label for the outside of the human erythrocyte membrane.
    Biochim Biophys Acta. 1969 Jun 3;183(1):65-78 PMID: 4183114
  13. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes.
    J Cell Biol. 1970 Apr;45(1):146-57 PMID: 5458993
  14. Initiation of haemoglobin synthesis by methionyl-tRNA.
    Nature. 1970 Aug 29;227(5261):913-8 PMID: 4915406
  15. Studies on glycopeptides released by trypsin from intact human erythrocytes.
    Biochemistry. 1967 Jul;6(7):2195-202 PMID: 6049454
  16. Evidence for an inhibitor in the control of globin synthesis by hemin in a reticulocyte lysate.
    Biochem Biophys Res Commun. 1969 Apr 10;35(1):79-85 PMID: 5779151
  17. A major protein which spans the human erythrocyte membrane.
    J Mol Biol. 1971 Jul 28;59(2):351-7 PMID: 5566420
  18. Control of globin synthesis in cell-free preparations of reticulocytes by formation of a translational repressor that is inactivated by hemin.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1565-8 PMID: 4504369
  19. Human erythrocyte membranes: specific labelling of surface proteins.
    J Mol Biol. 1971 Jun 28;58(3):775-81 PMID: 5091983
  20. An in vitro protein synthesizing system from mouse L fibroblasts infected with reovirus.
    Virology. 1971 Sep;45(3):724-33 PMID: 5125566
  21. The enzymatic iodination of the red cell membrane.
    J Cell Biol. 1972 Nov;55(2):390-405 PMID: 5076780
  22. Factors affecting the rate of protein synthesis in lysate systems from reticulocytes.
    Arch Biochem Biophys. 1968 May;125(2):671-83 PMID: 5656815
  23. In vitro synthesis of different categories of specific protein by membrane-bound and free ribosomes.
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1370-6 PMID: 5260940
  24. Major human erythrocyte glycoprotein spans the cell membrane.
    Nat New Biol. 1971 Jun 23;231(25):229-32 PMID: 5284359
  25. Alpha and beta globin messenger ribonucleic acid. Different amounts and rates of initiation of translation.
    J Biol Chem. 1971 Dec 10;246(23):7131-8 PMID: 5167019
  26. Hydroxylapatite chromatography of protein-sodium dodecyl sulfate complexes. A new method for the separation of polypeptide subunits.
    J Biol Chem. 1972 Aug 25;247(16):5194-8 PMID: 5066568
  27. Protein components of erythrocyte membranes from different animal species.
    Biochemistry. 1970 Dec 8;9(25):5037-40 PMID: 5480166
  28. Bacteriophage f2 RNA: control of translation and gene order.
    Nature. 1968 Oct 26;220(5165):345-50 PMID: 5684873
  29. Initiation of hemoglobin synthesis. Specific inhibition by antibiotics and bacteriophage ribonucleic acid.
    Biochemistry. 1971 Jun 8;10(12):2348-56 PMID: 5114993
  30. Exposed protein on the intact human erythrocyte.
    Biochemistry. 1971 May 11;10(10):1766-71 PMID: 5563761
  31. Studies on cessation of protein synthesis in a reticulocyte lysate cell-free system.
    Biochim Biophys Acta. 1970 Jul 16;213(1):237-40 PMID: 5488930
  32. A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions.
    J Biophys Biochem Cytol. 1960 Jul;7:619-30 PMID: 14446510
  33. Regulation of hemoglobin synthesis. Equal rates of translation and termination of - and -globin chains.
    J Biol Chem. 1972 Jun 10;247(11):3622-9 PMID: 4624122
  34. Membrane proteins.
    Annu Rev Biochem. 1972;41:731-52 PMID: 4263713
  35. Membranes of animal cells. II. The metabolism and turnover of the surface membrane.
    J Cell Biol. 1968 Jun;37(3):729-46 PMID: 11905204
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.5.1526
Published
1973-05-00
Pages
1526-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433535
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.5.1526 PMC Full Text View journal details
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