Isolation of messenger RNA coding for mouse heavy-chain immunoglobulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 4 ·1973-04-00 ·Pages 1127-31

Stevens RH, Williamson AR

Abstract

A method for purification of the mRNA coding for heavy-chain protein of a mouse G(2a) myeloma (5563) is reported. Complete myeloma protein (H(2)L(2)) specifically binds to the heavy-chain mRNA, and the resultant RNA-protein complex is precipitated with antiserum against 5563 myeloma protein. Cytoplasmic RNA isolated by this method showed two bands of 6.0 x 10(5) and 3.1 x 10(5) molecular weight when analyzed by acrylamide gel electrophoresis. The RNA in the bands contained 1.5% and 8% A-rich regions, respectively. The RNA in each of the bands contained sequences for heavy-chain protein when translated in Xenopus laevis oocytes.

MeSH Terms

Animals Antigen-Antibody Complex Antigen-Antibody Reactions Biological Assay Electrophoresis, Polyacrylamide Gel Female Immunoassay Immunoglobulin Fragments/biosynthesis Mice Myeloma Proteins/biosynthesis,metabolism Ovum/metabolism Phosphorus Isotopes Protein Binding Protein Biosynthesis Proteins/analysis RNA, Messenger/isolation & purification,metabolism Sulfur Isotopes Xenopus

Chemicals

Antigen-Antibody Complex Immunoglobulin Fragments Myeloma Proteins Phosphorus Isotopes Proteins RNA, Messenger Sulfur Isotopes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stevens R H

Williamson A R

References (15)

15 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.4.1127

Published

1973-04-00

Pages

1127-31

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433440

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.4.1127 PMC Full Text View journal details

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Isolation of messenger RNA coding for mouse heavy-chain immunoglobulin.

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