Restoration of hypoxanthine phosphoribosyl transferase activity in mouse 1R cells after fusion with chick-embryo fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 7 ·1973-07-00 ·Pages 1998-2002

Bakay B, Croce CM, Koprowski H, Nyhan WL

Abstract

Fusion of the 1R mouse cell, which lacks activity of hypoxanthine phosphoribosyl transferase (EC 2.4.2.8), with chick-embryo fibroblasts yielded progeny cells that survived in hypoxanthine-aminopterin-thymidine selective medium. This property and the failure of the progeny to survive in 8-azaguanine indicated that hypoxanthine phosphoribosyl transferase activity was present. Electrophoretic analysis revealed that the enzyme was of mouse, not chick, origin. These observations are consistent with the operation of a regulator gene responsible for the absence of hypoxanthine phosphoribosyl-transferase activity in the 1R cell and its presence in the progeny.

MeSH Terms

Adenine Aminopterin Animals Carbon Isotopes Cell Fusion Cell Line Chick Embryo Clone Cells Culture Media Electrophoresis, Polyacrylamide Gel Enzyme Activation Fibroblasts Glucosephosphate Dehydrogenase/analysis Hybrid Cells/enzymology Hypoxanthines Karyotyping L Cells Mice Pentosyltransferases/analysis,biosynthesis Thymidine

Chemicals

Carbon Isotopes Culture Media Hypoxanthines Glucosephosphate Dehydrogenase Pentosyltransferases Adenine Aminopterin Thymidine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bakay B

Croce C M

Koprowski H

Nyhan W L

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.7.1998

Published

1973-07-00

Pages

1998-2002

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433651

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.7.1998 PMC Full Text View journal details

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Restoration of hypoxanthine phosphoribosyl transferase activity in mouse 1R cells after fusion with chick-embryo fibroblasts.

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