Embryonic cell aggregation: kinetics and specificity of binding of enhancing factors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 3 ·1974-03-00 ·Pages 727-31

Balsamo J, Lilien J

Abstract

A new assay for tissue-type-specific adhesive macromolecules is described. This assay measures the binding to whole cells of radioactive macromolecules in aggregation-enhancing supernatants. We show for two tissue types, embryonic chick neural retina and cerebrum, that binding is tissue-type specific. Specific binding can be distinguished from nonspecific binding by its sensitivity to displacement with horse serum and by the kinetics of binding. Specific binding appears to be a cooperative phenomenon.

MeSH Terms

Animals Autoradiography Binding Sites Brain/cytology,embryology Cell Adhesion Cell Aggregation Cell Differentiation Cell Membrane Chick Embryo Glucosamine/metabolism Kinetics Liver/cytology,embryology Macromolecular Substances Retina/cytology,embryology Tritium

Chemicals

Macromolecular Substances Tritium Glucosamine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Balsamo J

Lilien J

References (7)

7 references, click to expand

Specific enhancement of cell aggregation in vitro.
Dev Biol. 1968 Jun;17(6):657-78 PMID: 5658965
Studies on intercellular adhesive selectivity.
Dev Biol. 1968 Dec;18(6):602-31 PMID: 5710082
Does differential adhesion govern self-assembly processes in histogenesis? Equilibrium configurations and the emergence of a hierarchy among populations of embryonic cells.
J Exp Zool. 1970 Apr;173(4):395-433 PMID: 5429514
Reconstruction of brain tissue from cell suspensions. 2. Specific enhancement of aggregation of embryonic cerebral cells by supernatant from homologous cell cultures.
Dev Biol. 1972 Feb;27(2):235-43 PMID: 5019937
Sialic acid and cell aggregation.
J Cell Sci. 1971 Nov;9(3):823-33 PMID: 5169782
A quantitative assay for intercellular adhesion.
Proc Natl Acad Sci U S A. 1973 May;70(5):1569-73 PMID: 4351182
Aggregation of embryonic cells in a serum-free medium and its inhibition at suboptimal temperatures.
Exp Cell Res. 1966 Mar;41(3):697-702 PMID: 4952062

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.3.727

Published

1974-03-00

Pages

727-31

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388086

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.3.727 PMC Full Text View journal details

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Embryonic cell aggregation: kinetics and specificity of binding of enhancing factors.

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