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PMID: 4522791 Published · ppublish English Journal Article

Requirement of initiation factor 3 in the initiation of polypeptide synthesis with N-acetylphenylalanyl-tRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 3 ·1974-03-00 ·Pages 774-8

Bernal SD, Blumberg BM, Nakamoto T

Abstract

Initiation factor 3 is required, along with initiation factors 1 and 2, for the incorporation of N-acetylphenylalanine into polypeptides and the formation of N-acetylphenylalanylpuromycin. Initiation factor 3 also strongly stimulates the binding of N-acetylphenylalanyl transfer RNA to isolated 30S ribosomal subunits. Phosphocellulose fractions of initiation factor 3 were found to catalyze N-acetylphenylalanine incorporation differentially with different synthetic messenger RNAs not containing any codons for N-formylmethionine. The results suggest that ribosomes recognize the initiator codon only through the initiator transfer RNA.

MeSH Terms
Binding Sites Carbon Radioisotopes Methionine/metabolism Peptide Chain Initiation, Translational Peptide Initiation Factors Phenylalanine Puromycin/metabolism RNA, Messenger/metabolism RNA, Transfer/metabolism Ribosomes/metabolism
Chemicals
Carbon Radioisotopes Peptide Initiation Factors RNA, Messenger Phenylalanine Puromycin RNA, Transfer Methionine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bernal S D
Blumberg B M
Nakamoto T
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.3.774
Published
1974-03-00
Pages
774-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388096
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.3.774 PMC Full Text View journal details
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