Polyadenylation of maternal RNA of sea urchin eggs after fertilization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 8 ·1973-08-00 ·Pages 2345-9

Wilt FH

Abstract

Between fertilization, or parthenogenetic activation, and the two-cell stage, the content of polyadenylic acid in sea urchin eggs doubles, and the increase occurs primarily in the ribosome-polyribosome fraction. The increase is due to polyadenylation of preexisting RNA molecules synthesized during oogenesis. The polyadenylation occurs in activated, enucleated merogons. It is argued that cytoplasmic polyadenylation may play a role in mobilization of maternal messenger RNA for translation and the polyadenylic acid does not subserve an exclusively nuclear function.

MeSH Terms

Adenine Nucleotides/analysis,metabolism Adenosine/metabolism Animals Cell Fractionation Centrifugation, Density Gradient Cytoplasm/metabolism Dactinomycin/pharmacology Female Fertilization Ovum/cytology,metabolism Polynucleotides/analysis,metabolism RNA/analysis,isolation & purification,metabolism Sea Urchins/metabolism Tritium

Chemicals

Adenine Nucleotides Polynucleotides Tritium Dactinomycin RNA Adenosine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Wilt F H

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.8.2345

Published

1973-08-00

Pages

2345-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433732

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.8.2345 PMC Full Text View journal details

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Polyadenylation of maternal RNA of sea urchin eggs after fertilization.

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