Home LiteratureArticle Details
PMID: 4525461 Published · ppublish English Journal Article

Distribution of repetitive and nonrepetivite sequence transcripts in HeLa mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 5 ·1974-05-00 ·Pages 1785-9

Klein WH, Murphy W, Attardi G, Britten RJ, Davidson EH

Abstract

Polyadenylated messenger RNA extracted from HeLa cells was hybridized with a mass excess of HeLa DNA. The kinetics of the hybridization reaction demonstrated that most of the messenger RNA is transcribed from nonrepetitive DNA. The amount of messenger RNA hybridized to DNA was measured both with and without prior RNase treatment. Comparison of the results indicates that within the limits of detection, HeLa messenger RNA does not contain repetitive sequence elements covalently linked to nonrepetitive sequence transcripts. However, a small fraction of the HeLa messenger RNA preparation is transcribed entirely from repetitive DNA sequences. This fraction represents about 6% of the total polyadenylated messenger RNA preparation.

MeSH Terms
Adenosine Base Sequence Chromatography DNA Female Genetic Code HeLa Cells/analysis,metabolism Humans Hydroxyapatites Kinetics Nucleic Acid Hybridization Polynucleotides RNA, Messenger/analysis RNA, Neoplasm/analysis Transcription, Genetic
Chemicals
Hydroxyapatites Polynucleotides RNA, Messenger RNA, Neoplasm DNA Adenosine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Klein W H
Murphy W
Attardi G
Britten R J
Davidson E H
References (20)
20 references, click to expand
  1. The distribution of rapidly hybridizing RNA sequences in heterogeneous nuclear RNA and mRNA from HeLa cells.
    J Cell Physiol. 1970 Dec;76(3):349-56 PMID: 5502353
  2. Electron microscopic visualization of mitochondrial RNA-DNA hybrids.
    J Mol Biol. 1971 Jan 28;55(2):267-70 PMID: 5548608
  3. Reiteration and clustering of DNA sequences complementary to histone messenger RNA.
    Nat New Biol. 1971 Apr 7;230(14):165-9 PMID: 5279989
  4. DNA-RNA hybridization in vast DNA excess.
    Nat New Biol. 1971 May 5;231(18):8-12 PMID: 4932893
  5. Hybridization properties of DNA sequences directing the synthesis of messenger RNA and heterogeneous nuclear RNA.
    J Cell Biol. 1971 Sep;50(3):774-86 PMID: 4999767
  6. Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehyde.
    J Mol Biol. 1968 Jul 14;35(1):61-70 PMID: 4939779
  7. Reiteration frequency of haemoglobin genes in the duck.
    Nat New Biol. 1972 Feb 23;235(60):231-4 PMID: 4502410
  8. Evidence for single copies of globin genes in the mouse genome.
    Nature. 1972 Sep 22;239(5369):219-21 PMID: 4117010
  9. The genes for silk fibroin in Bombyx mori.
    J Mol Biol. 1972 Oct 14;70(3):637-49 PMID: 5083150
  10. Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum.
    Nat New Biol. 1972 Oct 25;239(95):225-8 PMID: 4507801
  11. Stability of cytoplasmic messenger RNA in HeLa cels.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):115-9 PMID: 4509644
  12. Genes coding for polysomal 9S RNA of sea urchins: conservation and divergence.
    Nature. 1972 Nov 24;240(5378):225-8 PMID: 4569419
  13. Reiteration frequency of duck haemoglobin genes.
    Nat New Biol. 1973 Feb 14;241(111):204-7 PMID: 4512585
  14. Hybridization properties and sequence arrangement in a population of mRNAs.
    Nat New Biol. 1973 Mar 28;242(117):101-5 PMID: 4513407
  15. Renaturation of bacteriophage phiX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant.
    J Mol Biol. 1973 Jul 15;77(4):495-500 PMID: 4579448
  16. Synthesis of a deoxyribonucleic acid sequence complementary to ovalbumin messenger ribonucleic acid and quantification of ovalbumin genes.
    J Biol Chem. 1973 Nov 10;248(21):7530-9 PMID: 4745779
  17. Nonrepetitive DNA sequence representation in sea urchin embryo messenger RNA.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3516-20 PMID: 4519642
  18. Hydroxyapatite chromatography of short double-helical DNA.
    Biochim Biophys Acta. 1973 Dec 21;331(3):333-40 PMID: 4777662
  19. Organization, transcription, and regulation in the animal genome.
    Q Rev Biol. 1973 Dec;48(4):565-613 PMID: 4592421
  20. A simplified procedure for purification of large amounts of poliovirus: characterization and amino acid analysis of Type 1 poliovirus.
    J Biol Chem. 1960 Jan;235:70-3 PMID: 14416320
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.5.1785
Published
1974-05-00
Pages
1785-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388325
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.5.1785 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2