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PMID: 4526209 Published · ppublish English Journal Article

Rapid enhancement of chick intestinal DNA-dependent RNA polymerase II activity by 1 alpha, 25-dihydroxyvitamin D3, in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 6 ·1974-06-00 ·Pages 2337-41

Zerwekh JE, Haussler MR, Lindell TJ

Abstract

1alpha,25-dihydroxyvitamin D(3) was examined for its ability to affect the DNA-dependent RNA polymerases (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) of rachitic chick intestinal cell nuclei in vivo. Nucleoplasmic (form II) RNA polymerase activity was stimulated 2-fold (P < 0.05) within 2-3 hr after an oral dose of 0.27 mug (0.65 nmol) of 1alpha,25-dihydroxyvitamin D(3) to rachitic chicks. The form II polymerase activity returned to control values by 5-9 hr after dosing with the sterol. In contrast, the nucleolar (form I) RNA polymerase was not increased within this period. Solubilization of nuclear protein and resolution of the two RNA polymerases on DEAE-Sephadex also revealed that there was an increase in polymerase II activity when assayed on exogenous DNA template. This evidence suggests that 1alpha,25-dihydroxyvitamin D(3) acts at the level of the enzymology of intestinal cell transcription and that increased mRNA synthesis after administration of this hormone cannot be due solely to a change in chromatin template activity.

MeSH Terms
Animals Calcium/metabolism Cell Nucleus/enzymology Chickens Chromatin Chromatography, Ion Exchange DNA-Directed RNA Polymerases/metabolism Dihydroxycholecalciferols/metabolism,pharmacology Hydroxycholecalciferols/pharmacology Intestinal Absorption Intestinal Mucosa/enzymology Intestine, Small/enzymology Male RNA, Messenger/biosynthesis Rickets/enzymology Stimulation, Chemical Templates, Genetic Transcription, Genetic/drug effects Tritium
Chemicals
Chromatin Dihydroxycholecalciferols Hydroxycholecalciferols RNA, Messenger Tritium DNA-Directed RNA Polymerases Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zerwekh J E
Haussler M R
Lindell T J
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.6.2337
Published
1974-06-00
Pages
2337-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388449
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.6.2337 PMC Full Text View journal details
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