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PMID: 4527822 Published · ppublish English Journal Article

Growth control in cultured mouse fibroblasts: induction of the pleiotypic and mitogenic responses by a purified growth factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 7 ·1974-07-00 ·Pages 2600-4

Rudland PS, Seifert W, Gospodarowicz D

Abstract

Addition of serum to quiescent mouse fibroblasts induces a series of macromolecular changes (a pleiotypic response) followed by DNA synthesis and cell division. A new pituitary hormone, fibroblast growth factor, and hydrocortisone acting at physiological concentrations can completely replace exogenously added serum for the induction of these events in lines of BALB/c 3T3 cells. The induction of cell growth is specific for cultured fibroblasts; no stimulation is observed for mouse epithelial cells or virally transformed fibroblasts.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic DNA/biosynthesis Fibroblasts/drug effects,metabolism,physiology Hydrocortisone/pharmacology Mice Mice, Inbred BALB C Mitosis/drug effects Pituitary Hormones/pharmacology Protein Biosynthesis RNA/biosynthesis Stimulation, Chemical Thymidine/metabolism Time Factors Tritium Uridine/metabolism
Chemicals
Pituitary Hormones Tritium RNA DNA Thymidine Uridine Hydrocortisone
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rudland P S
Seifert W
Gospodarowicz D
References (21)
21 references, click to expand
  1. The initiation of cell division in a contact-inhibited mammalian cell line.
    J Cell Physiol. 1965 Dec;66(3):325-33 PMID: 5884360
  2. Studies on carcinogenesis by avian sarcoma viruses. VI. Differential multiplication of uninfected and of converted cells in response to insulin.
    J Cell Physiol. 1967 Jun;69(3):377-84 PMID: 4296516
  3. "Contact inhibition" of cell division in 3T3 cells.
    Proc Natl Acad Sci U S A. 1968 May;60(1):300-4 PMID: 5241531
  4. Topoinhibition and serum requirement of transformed and untransformed cells.
    Nature. 1970 Aug 22;227(5260):802-6 PMID: 4317354
  5. Serum factor requirements of normal and simian virus 40-transformed 3T3 mouse fibroplasts.
    Proc Natl Acad Sci U S A. 1971 Mar;68(3):645-52 PMID: 5276775
  6. "Pleiotypic response".
    Nat New Biol. 1971 Aug;232(33):206-11 PMID: 5285753
  7. Detection of polyadenylic acid sequences in viral and eukaryotic RNA(polu(U)-cellulose columns-poly(U) filters-fiberglass-HeLa cells-bacteriophage T4).
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):417-21 PMID: 4501121
  8. Insulin receptors in human circulating cells and fibroblasts.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):747-51 PMID: 4501590
  9. Difference in the cyclic adenosine 3',5'-monophosphate levels in normal and transformed cells.
    Nat New Biol. 1972 Mar 1;236(61):14-6 PMID: 4336394
  10. Regulation of cell growth by cyclic adenosine 3',5'-monophosphate. Effect of cell density and agents which alter cell growth on cyclic adenosine 3',5'-monophosphate levels in fibroblasts.
    J Biol Chem. 1972 Nov 10;247(21):7082-7 PMID: 4343168
  11. Pituitary extracts and steroid hormones in the control of 3T3 cell growth.
    Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702-6 PMID: 4354860
  12. Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2964-8 PMID: 4355377
  13. Cyclic GMP and cell movement.
    Nature. 1973 Oct 26;245(5426):458-60 PMID: 4356150
  14. Role of insulin as a "permissive" hormone in mammary gland development.
    Nature. 1973 Nov 16;246(5429):159-60 PMID: 4586108
  15. Conditions limiting multiplication of fibroblastic and epithelial cells in dense cultures.
    Nature. 1973 Nov 23;246(5430):197-9 PMID: 4586792
  16. Kinetics of early changes in phosphate and uridine transport and cyclic AMP levels stimulated by serum in density-inhibited 3T3 cells.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):96-8 PMID: 4359335
  17. Possible involvement of cyclic GMP in growth control of cultured mouse cells.
    Nature. 1974 Mar 8;248(5444):138-40 PMID: 4362085
  18. Control of translation in cultured cells: continued synthesis and accumulation of messenger RNA in nondividing cultures.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):750-4 PMID: 4207072
  19. Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth.
    Nature. 1974 May 10;249(453):123-7 PMID: 4364816
  20. Purification of a growth factor for ovarian cells from bovine pituitary glands.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2295-9 PMID: 4526208
  21. CONTACT INHIBITION, MACROMOLECULAR SYNTHESIS, AND POLYRIBOSOMES IN CULTURED HUMAN DIPLOID FIBROBLASTS.
    Proc Natl Acad Sci U S A. 1965 Feb;53:350-6 PMID: 14294068
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.7.2600
Published
1974-07-00
Pages
2600-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388513
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.7.2600 PMC Full Text View journal details
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