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PMID: 4528509 Published · ppublish English Journal Article

Differentiation between intracellular and cell surface glycosyl transferases: galactosyl transferase activity in intact cells and in cell homogenate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3068-72

Deppert W, Werchau H, Walter G

Abstract

Intact BHK (baby hamster kidney) cells catalyze the hydrolysis of UDP-galactose to free galactose. The generation of galactose from UDP-galactose and its intracellular utilization impede the detection of possible galactosyl transferases on the cell surface of intact cells. Several independent procedures have been used to distinguish between intracellular and cell surface glycosyl transferases. With these procedures, no evidence was obtained for the presence of detectable amounts of galactosyl transferase activity on the surface of BHK cells. The data suggest that galactosyl transferases do not play a general role in the phenomena of cell adhesion and contact inhibition.

MeSH Terms
Animals Carbon Radioisotopes Cell Adhesion Cell Line Cell Membrane/enzymology Chromatography, Paper Contact Inhibition Cricetinae Galactose Glucosyltransferases/metabolism Hexosyltransferases/metabolism Hydrolysis Kidney Magnesium/metabolism Tritium Uridine Diphosphate Sugars/metabolism
Chemicals
Carbon Radioisotopes Uridine Diphosphate Sugars Tritium Glucosyltransferases Hexosyltransferases Magnesium Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Deppert W
Werchau H
Walter G
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.8.3068
Published
1974-08-00
Pages
3068-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388622
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.8.3068 PMC Full Text View journal details
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