Free ribosomal RNA genes in the macronucleus of Tetrahymena.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3078-81

Gall JG

Abstract

In the macronucleus of the ciliated protozoan, Tetrahymena pyriformis GL, the genes coding for 17S and 25S rRNA exist as free, extrachromosomal molecules. About 90% of the molecules are linear with a molecular weight of 12.6 x 10(6). Most of the remainder are circles of the same size, or lariats (circles with tails). A few dimers and oligomers are found. The extrachromosomal rDNA of Tetrahymena represents a kind of gene amplification that may be common among primitive eukaryotes.

MeSH Terms

Animals Cell Nucleus/analysis,metabolism Centrifugation, Density Gradient DNA/analysis DNA Replication DNA, Circular/analysis Extrachromosomal Inheritance Genes Microscopy, Electron Nucleic Acid Denaturation Nucleic Acid Hybridization Phosphorus Radioisotopes RNA, Ribosomal/biosynthesis,metabolism Tetrahymena/analysis Tritium Uridine/metabolism

Chemicals

DNA, Circular Phosphorus Radioisotopes RNA, Ribosomal Tritium DNA Uridine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Gall J G

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.8.3078

Published

1974-08-00

Pages

3078-81

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388624

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.8.3078 PMC Full Text View journal details

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Free ribosomal RNA genes in the macronucleus of Tetrahymena.

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