Role for heme in mammalian protein synthesis: activation of an initiation factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 10 ·1974-10-00 ·Pages 4020-24

Raffel C, Stein S, Kaempfer R

Abstract

Two nonerythropoietic tissues, brian and liver, contain an initiation factor that can overcome the block in initiation of protein synthesis seen in reticulocyte lysates when exogenous hemin is not present. Upon incubation of the brain factor with hemin and removal of free hemin by gel filtration, the factor activity is strongly stimulated. This stimulation shows a concentration dependence on hemin close to that seen for stimulation of protein synthesis in whole reticulocyte lysates. The data indicate that hemin mediates the formation of an active initiation factor complex from inactive, lower molecular weight components.

MeSH Terms

Animals Brain Chemistry Cell-Free System Chromatography, Gel Heme/pharmacology,physiology Kinetics Liver/analysis Mice Peptide Initiation Factors/isolation & purification Polyribosomes/metabolism Protein Biosynthesis Rabbits Rats Reticulocytes/metabolism,ultrastructure Stimulation, Chemical

Chemicals

Peptide Initiation Factors Heme

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Raffel C

Stein S

Kaempfer R

References (10)

10 references, click to expand

Stimulation of globin-chain initiation by hemin in the reticulocyte cell-free system.
Proc Natl Acad Sci U S A. 1968 Feb;59(2):582-9 PMID: 5238986
Factors affecting the rate of protein synthesis in lysate systems from reticulocytes.
Arch Biochem Biophys. 1968 May;125(2):671-83 PMID: 5656815
Effects of hemin and other porphyrins on protein synthesis in a reticulocyte lysate cell-free system.
J Mol Biol. 1969 Jun 14;42(2):247-58 PMID: 5803298
Translational control in hemoglobin syntheskis.
Cold Spring Harb Symp Quant Biol. 1969;34:567-78 PMID: 5266178
Control of globin synthesis: the role of heme.
J Mol Biol. 1972 May 28;66(3):471-81 PMID: 5037023
Translational control of hemoglobin synthesis by an initiation factor required for recycling of ribosomes and for their binding to messenger RNA.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3317-21 PMID: 4508325
Ovalbumin messenger ribonucleic acid translation. Comparable rates of polypeptide initiation and elongation on ovalbumin and globin messenger ribonucleic acid in a rabbit reticulocyte lysate.
J Biol Chem. 1973 Mar 25;248(6):2095-106 PMID: 4690593
Effect of hemin on the synthesis of hemoglobin and other proteins in mammalian cells.
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1022-6 PMID: 4197926
Inhibition of cellular protein synthesis by double-stranded RNA: inactivation of an initiation factor.
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1222-6 PMID: 4197930
Influence of prosthetic groups on protein folding and subunit assembly. Recombination of separated human alpha-and beta-globin chains with heme and alloplex interactions of globin chains with heme-containing subunits.
J Biol Chem. 1973 Sep 25;248(18):6462-70 PMID: 4730328

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.10.4020

Published

1974-10-00

Pages

4020-24

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC434319

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.10.4020 PMC Full Text View journal details

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Role for heme in mammalian protein synthesis: activation of an initiation factor.

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