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PMID: 4530305 Published · ppublish English Journal Article

Purification of naked intranuclear particles from human liver infected by hepatitis B virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3345-9

Hirschman SZ, Gerber M, Garfinkel E

Abstract

The isolation and purification of the naked intranuclear particles of a human liver infected with hepatitis B virus is described. The particles had a diameter of 27-28 nm, were hexagonal, and showed the presence of capsomeres and spikes with lengths of 7-10 nm projecting from the surfaces. The particles formed cubic arrays with center-to-center distances of 40-42 nm. The overall particle symmetry was icosahedral. The ultraviolet absorption spectrum of the particles showed a peak at 264 nm with a shoulder at 280 nm. Endogenous DNA polymerase activity was not detected in fractions containing particles. DNA polymerase activity was present when activated DNA or d(A-T)(n) was used as exogenous template.

MeSH Terms
Absorption Cell Fractionation Cell Nucleus/enzymology,microbiology Centrifugation, Density Gradient DNA Nucleotidyltransferases/analysis DNA-Directed RNA Polymerases/analysis Hepatitis A/microbiology Hepatitis B Antigens Hepatitis Viruses/isolation & purification Humans Liver/microbiology,ultrastructure Microscopy, Electron Polynucleotides Spectrophotometry, Ultraviolet Templates, Genetic
Chemicals
Hepatitis B Antigens Polynucleotides DNA Nucleotidyltransferases DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hirschman S Z
Gerber M
Garfinkel E
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.9.3345
Published
1974-09-00
Pages
3345-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433768
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.9.3345 PMC Full Text View journal details
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