The 7S RNA common to oncornaviruses and normal cells is associated with polyribosomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3390-4

Walker TA, Pace NR, Erikson RL, Erikson E, Behr F

Abstract

The 7S RNA species first demonstrated in avian and murine oncornaviruses and later in normal, uninfected cells is found associated in part with cellular polyribosomes. A molar ratio of 7S RNA to 5S ribosomal RNA of 0.05 indicates that there is approximately one mole of 7S RNA per mole of messenger RNA. Dissociation of polyribosomes with dimethylsulfoxide results in a marked decrease in the sedimentation rate of the 7S RNA. The dimethylsulfoxide-induced dissociation of polyribosomes and the concomitant movement of the 7S RNA from the polyribosome region into lighter regions of a sucrose gradient are both inhibited by cycloheximide, indicating that the 7S RNA is indeed associated with polyribosomes and not with a ribonucleoprotein particle sedimenting with polyribosomes.

MeSH Terms

Animals Cell Fractionation Centrifugation, Density Gradient Chromatography Cycloheximide/pharmacology Dimethyl Sulfoxide Electrophoresis, Polyacrylamide Gel Fibroblasts/analysis HeLa Cells/analysis Humans L Cells/analysis Mice Molecular Weight Oncogenic Viruses/analysis Polyribosomes/analysis RNA Viruses/analysis RNA, Neoplasm/analysis RNA, Ribosomal/analysis RNA, Viral/analysis Subcellular Fractions

Chemicals

RNA, Neoplasm RNA, Ribosomal RNA, Viral Cycloheximide Dimethyl Sulfoxide

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Walker T A

Pace N R

Erikson R L

Erikson E

Behr F

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27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.9.3390

Published

1974-09-00

Pages

3390-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433778

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.9.3390 PMC Full Text View journal details

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The 7S RNA common to oncornaviruses and normal cells is associated with polyribosomes.

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