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PMID: 4530316 Published · ppublish English Journal Article

A neuroblastoma times glioma hybrid cell line with morphine receptors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3474-7

Klee WA, Nirenberg M

Abstract

A neuroblastoma x glioma hybrid cell line with well-developed neural properties was found that has high-affinity morphine receptors. The average cell contains approximately 3 x 10(6) receptors. In contrast, parent cells and other neuroblastoma or hybrid cell lines tested had few or no morphine receptors.

MeSH Terms
Animals Cell Line Glioma/metabolism Hybrid Cells Mice Morphine/metabolism Morphine Derivatives Naloxone/metabolism Nerve Tissue Proteins/metabolism Neuroblastoma/metabolism Rats Receptors, Drug Tritium
Chemicals
Morphine Derivatives Nerve Tissue Proteins Receptors, Drug Tritium Naloxone Morphine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klee W A
Nirenberg M
References (18)
18 references, click to expand
  1. Kinetic parameters of narcotic agonists and antagonists, with particular reference to N-allylnoroxymorphone (naloxone).
    Br J Pharmacol Chemother. 1968 Jun;33(2):266-76 PMID: 5664147
  2. Genes for neuronal properties expressed in neuroblastoma x L cell hybrids.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):234-9 PMID: 5276295
  3. Stereospecific and nonspecific interactions of the morphine congener levorphanol in subcellular fractions of mouse brain.
    Proc Natl Acad Sci U S A. 1971 Aug;68(8):1742-7 PMID: 5288759
  4. Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase-acetylcholinesterase-tyrosine hydroxylase-axons-dendrites).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):258-63 PMID: 4400294
  5. Genetic dissection of neural properties using somatic cell hybrids.
    Nat New Biol. 1972 Feb 23;235(60):225-31 PMID: 4111970
  6. Opiate receptor: demonstration in nervous tissue.
    Science. 1973 Mar 9;179(4077):1011-4 PMID: 4687585
  7. -Bungarotoxin used as a probe for acetylcholine receptors of cultured neurones.
    Nature. 1973 May 18;243(5403):163-6 PMID: 4550035
  8. Stereospecific binding of the potent narcotic analgesic (3H) Etorphine to rat-brain homogenate.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):1947-9 PMID: 4516196
  9. Regional distribution of opiate receptor binding in monkey and human brain.
    Nature. 1973 Oct 26;245(5426):447-50 PMID: 4127185
  10. Opiate agonists and antagonists discriminated by receptor binding in brain.
    Science. 1973 Dec 28;182(4119):1359-61 PMID: 4128222
  11. Distribution of stereospecific binding of the potent narcotic analgesic etorphine in the human brain: predominance in the limbic system.
    Res Commun Chem Pathol Pharmacol. 1973 Nov;6(3):1052-62 PMID: 4760880
  12. Stereospecific interaction of opiate narcotics in binding of 3H-dihydromorphine to membranes of rat brain.
    Life Sci. 1973 Dec 1;13(11):1543-56 PMID: 4768981
  13. Stereospecific interaction between narcotic analgesics and a synaptic plasm a membrane fraction of rat cerebral cortex.
    Acta Pharmacol Toxicol (Copenh). 1973;32(3):317-20 PMID: 4801733
  14. Morphine-like drugs inhibit the stimulation of E prostaglandins of cyclic AMP formation by rat brain homogenate.
    Nature. 1974 Mar 1;248(5443):24-7 PMID: 4361995
  15. Narcotic receptor sites in morphine-dependent rats.
    Nature. 1974 Mar 1;248(5443):61-3 PMID: 4131940
  16. Properties of opiate-receptor binding in rat brain.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2243-7 PMID: 4525427
  17. Synthetic substances with morphine-like effect: clinical experience; potency, side-effects, addiction liability.
    Bull World Health Organ. 1957;17(4-5):569-863 PMID: 13511135
  18. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS.
    Anal Chem. 1965 Jun;37:854-7 PMID: 14326976
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.9.3474
Published
1974-09-00
Pages
3474-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433796
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.9.3474 PMC Full Text View journal details
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