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PMID: 4531028 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis of a pentekontapeptide with high lipolytic activity corresponding to the carboxyl-terminal fifty amino acids of ovine beta-lipotropin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 4945-9

Yamashiro D, Li CH

Abstract

The synthesis of a peptide, composed of fifty amino-acid residues, corresponding to positions 42-91 in ovine beta-lipotropin has been accomplished by the solidphase method. The preformed symmetrical anhydride and active ester coupling methods were used exclusively. The synthetic product was purified by gel filtration, carboxymethylcellulose chromatography, and partition chromatography on Sephadex G-50. Its lipolytic activity in isolated rabbit fat cells was about six times that of beta-lipotropin on a weight basis.

MeSH Terms
Adipose Tissue/metabolism Amino Acid Sequence Animals Biological Assay Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Peptide Fragments/chemical synthesis Rabbits Sheep beta-Lipotropin/chemical synthesis,isolation & purification
Chemicals
Peptide Fragments beta-Lipotropin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yamashiro D
Li C H
References (29)
29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.4945
Published
1974-12-00
Pages
4945-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434016
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.4945 PMC Full Text View journal details
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