Home LiteratureArticle Details
PMID: 4531030 Published · ppublish English Journal Article

Endonuclease activity associated with purified PM2 bacteriophages.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 4965-9

Laval F

Abstract

An endonucleolytic activity is associated with purified PM(2) bacteriophages. It converts the double-stranded supercoiled PM(2) DNA mostly into the linear form that sediments as a homogenous peak in alkaline sucrose gradients. The same activity is found in intact or detergent-lysed phages. Optimal activity is observed between pH 6.8 and 7.5 at 28 degrees . Divalent cations, Mg(2+) or Mn(2+), are necessary for activity, and the enzyme is inhibited by RNA. The endonuclease has no appreciable activity on linear DNA molecules, but it attacks single-stranded circular DNA.

MeSH Terms
Bacteriophages/enzymology Carbon Radioisotopes Centrifugation, Density Gradient DNA, Circular/metabolism DNA, Viral/metabolism Endonucleases/metabolism Hydrogen-Ion Concentration Microscopy, Electron Nucleic Acid Conformation Pseudomonas Thymidine/metabolism Tritium
Chemicals
Carbon Radioisotopes DNA, Circular DNA, Viral Tritium Endonucleases Thymidine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Laval F
References (22)
22 references, click to expand
  1. Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1300-6 PMID: 4870862
  2. Properties of bacteriophage PM2: a lipid-containing bacterial virus.
    Virology. 1968 Apr;34(4):738-47 PMID: 5655723
  3. DNA of bacteriophage PM2: a closed circular double-stranded molecule.
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164-8 PMID: 5260915
  4. Structure and synthesis of a lipid-containing bacteriophage. 3. Purification of bacteriophage PM2 and some structural studies on the virion.
    Virology. 1969 Dec;39(4):666-81 PMID: 5358823
  5. Heterologous DNA nuclear uptake by mouse DNA fibroblasts in vitro and its early fate.
    Exp Cell Res. 1970 Nov;63(1):69-77 PMID: 4924802
  6. Replication of bacteriophage PM2 deoxyribonucleic acid: a closed circular double-stranded molecule.
    J Mol Biol. 1971 Mar 28;56(3):597-621 PMID: 5573015
  7. Structure and synthesis of a lipid-containing bacteriophage. VII. Structural proteins of bacteriophage PM2.
    Virology. 1971 Jul;45(1):232-9 PMID: 5112412
  8. [Endonucleasic activity of purified preparations of Polyoma virus].
    C R Acad Sci Hebd Seances Acad Sci D. 1971 Dec 20;273(25):2650-3 PMID: 4334536
  9. Structure and synthesis of a lipid-containing bacteriophage. X. Improved techniques for the purification of bacteriophage PM2.
    Virology. 1972 Apr;48(1):259-62 PMID: 5017150
  10. Use of ethidium bromide for separation and determination of nucleic acids of various conformational forms and measurement of their associated enzymes.
    Methods Biochem Anal. 1971;20:41-86 PMID: 4947800
  11. DNA-dependent RNA polymerase associated with bacteriophage PM2.
    Nat New Biol. 1972 Apr 5;236(66):131-2 passim PMID: 4502814
  12. Endonuclease activity associated with purified simian virus 40 virions.
    J Virol. 1972 May;9(5):800-3 PMID: 4337165
  13. Replicating, convalently closed, circular DNA from kinetoplasts of Trypanosoma cruzi.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1642-6 PMID: 4556467
  14. Purification and properties of an acid deoxyribonuclease from the snail Helix aspersa Müll.
    Biochemistry. 1972 Sep 12;11(19):3596-603 PMID: 5053762
  15. Mechanism of deoxyribonucleic acid degradation by an acid deoxyribonuclease from the snail Helix aspersa Müll.
    Biochemistry. 1972 Sep 12;11(19):3604-10 PMID: 4559795
  16. Characterization of an endonuclease associated with simian virus 40 virions.
    J Virol. 1972 Sep;10(3):410-6 PMID: 4342049
  17. Action of the S1 endonuclease from Aspergillus oryzae on simian virus 40 supercoiled component I DNA.
    Biochem Biophys Res Commun. 1973 Oct 15;54(4):1306-20 PMID: 4356815
  18. Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549-53 PMID: 4598307
  19. Characterization of the single-strand-specific nuclease S1 activity on double-stranded supercoiled polyoma DNA.
    Eur J Biochem. 1974 Apr 16;43(3):591-600 PMID: 4364862
  20. Endonucleolytic cleavage of polyoma virus DNA: general properties and site specificity of the virion-associated endonuclease.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:247-54 PMID: 239808
  21. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  22. A rapidly labeled RNA in rat liver nuclei.
    J Mol Biol. 1962 Aug;5:217-29 PMID: 13954349
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.4965
Published
1974-12-00
Pages
4965-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434020
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.4965 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2