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PMID: 4531036 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Proton-enhanced 13C nuclear magnetic resonance of lipids and biomembranes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 5062-7

Urbina J, Waugh JS

Abstract

A recently developed nuclear double resonance technique which permits sensitive detection, together with high resolution, of rare spins in solids or other dipolar-coupled nuclear systems [Pines, Gibby, and Waugh (1973) J. Chem. Phys. 59, 569] has been applied to the study of natural abundance (13)C-nuclear magnetic resonance in lipid mesophases and of selectively labeled carbon sites in bacterial membranes.Detailed microscopic information on the molecular organization and phase transitions of the lipid phases and their interaction with ions and other molecules can be obtained from the study of the chemical shift anisotropies and dynamical aspects of the (13)C NMR spectra of unsonicated lipid dispersions (liposomes). Experiments are reported which demonstrated the feasibility of quantitatively observing the (13)C-nuclear magnetic resonance of specifically labeled sites in unperturbed Escherichia coli membrane vesicles for the study of the physical state of the lipids with the aim of relating it to the known lipid-dependent functional properties of the membranes.

MeSH Terms
Carbon Isotopes Lipids Liposomes Magnetic Resonance Spectroscopy/methods Membranes, Artificial Phosphatidylcholines Protons
Chemicals
Carbon Isotopes Lipids Liposomes Membranes, Artificial Phosphatidylcholines Protons
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Urbina J
Waugh J S
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.5062
Published
1974-12-00
Pages
5062-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC434040
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.5062 PMC Full Text View journal details
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