The presence of 5-hydroxymethylcytosine in animal deoxyribonucleic acid.
A method is given for small-scale preparation of DNA from 1.0-1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytosine is also demonstrable in mouse and frog brain DNA and in the crude cytidylic acid fractions obtained from RNA hydrolysates of rat brain and liver. 5-Hydroxymethylcytosine is identified by paper chromatography, u.v. spectra in acid and alkaline solutions and by its conversion into 5-hydroxymethyluracil.
17 references, click to expand
-
The lipid composition of purified bovine brain myelin.J Neurochem. 1966 Apr;13(4):213-22 PMID: 5937889
-
Comparison of the DNA's obtained from brain nuclei and mitochondria of mice and from the nuclei and kinetoplasts of Leishmania enriettii.Biochim Biophys Acta. 1966 Aug 17;123(2):298-305 PMID: 5970343
-
Composition of deoxyribonucleoprotein in human brain.Clin Chim Acta. 1966 Oct;14(4):429-34 PMID: 4961662
-
Effect of divalent cations on the reduction and re-formation of the disulfide bonds of deoxyribonuclease.J Biol Chem. 1969 Feb 10;244(3):929-32 PMID: 4976791
-
Quantitative determination of deoxyribonucleic acid in rat brain.Biochem J. 1969 Nov;115(3):563-8 PMID: 5353529
-
Detection of sugars on paper chromatograms.Nature. 1950 Sep 9;166(4219):444-5 PMID: 14775715
-
The purine and pyrimidine composition of deoxypentose nucleic acids.Biochem J. 1951 May;48(5):584-90 PMID: 14838906
-
The composition of the deoxyribonucleic acid of salmon sperm.J Biol Chem. 1951 Sep;192(1):223-30 PMID: 14917668
-
Spectrophotometric studies of nucleic acid derivatives and related compounds as a function of pH. I. Pyrimidines.Biochim Biophys Acta. 1952;9(2):199-218 PMID: 12977804
-
Nucleic acid economy in bacteria infected with bacteriophage T2. I. Purine and pyrimidine composition.J Gen Physiol. 1953 Jul;36(6):777-89 PMID: 13069681
-
The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine.Biochem J. 1953 Dec;55(5):774-82 PMID: 13115372
-
The origin of purine and pyrimidine deoxyribose in T6r+ bacteriophage.J Biol Chem. 1959 Feb;234(2):364-9 PMID: 13630912
-
THE DETERMINATION OF DEOXYRIBONUCLEIC ACID AND OF CELL NUMBER IN BRAIN.J Neurochem. 1964 Jul;11:505-9 PMID: 14200773
-
Chromatographic studies of nucleic acids; a technique for the identification and estimation of purine and pyrimidine bases, nucleosides and related substances.Biochem J. 1949;45(3):294-8 PMID: 15392854
-
Separation of deoxyribonucleic acid from ribonucleic acid without the use of enzymes.Biochim Biophys Acta. 1961 Feb 12;47:18-26 PMID: 13830959
-
Deoxyribonucleic acids of central nervous system, kidney, and spleen: a comparison of some chemical and physical properties.J Neurochem. 1960 May;5:236-44 PMID: 13820143
-
Improved separation of sugars on the paper partition chromatogram.Biochem J. 1949;44(4):402-7 PMID: 16748536
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