An upper limit to the number of sodium channels in frog skin epithelium.

The Journal of physiology ·Vol. 228 ·No. 3 ·1973-02-00 ·Pages 681-92

Cuthbert AW

Abstract

1. Binding of [(14)C]amiloride to the mucosal surface of frog skin epithelium has been measured in the presence and absence of an excess of unlabelled amiloride. Simultaneous records were obtained of the sodium transport through the skin as indicated by the short circuit current.2. The number of binding sites with affinities for amiloride of 5 x 10(7) l./mole was around 400/mum(2).3. The relation between the binding sites and sodium channels is discussed.4. Making certain assumptions it is concluded that when frog skin epithelium is bathed in Ringer solution each sodium channel can handle 8000 sodium ions/sec.

MeSH Terms

Abdomen Amiloride/administration & dosage,metabolism,pharmacology Animals Anura Binding Sites Biological Transport, Active Carbon Isotopes Depression, Chemical Dose-Response Relationship, Drug Epithelium/metabolism In Vitro Techniques Membrane Potentials Rana temporaria Skin/metabolism Skin Physiological Phenomena Sodium/metabolism

Chemicals

Carbon Isotopes Amiloride Sodium

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Cuthbert A W

References (15)

15 references, click to expand

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Article Info

Journal

The Journal of physiology

Abbr.

J Physiol

ISSN

0022-3751

DOI

10.1113/jphysiol.1973.sp010106

Published

1973-02-00

Pages

681-92

Language

English

Region

England

NLM ID

0266262

PMCID

PMC1331246

Subset

External Links

PubMed source DOI: 10.1113/jphysiol.1973.sp010106 PMC Full Text View journal details

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An upper limit to the number of sodium channels in frog skin epithelium.

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