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PMID: 4542253 Published · ppublish English Journal Article

Complement-dependent release of immune complexes from the lymphocyte membrane.

The Journal of experimental medicine ·Vol. 138 ·No. 3 ·1973-09-01 ·Pages 495-507

Miller GW, Saluk PH, Nussenzweig V

Abstract

Soluble antigen-antibody-complement complexes bound to mouse B lymphocytes are rapidly released from the cell membrane in the presence of normal serum from several mammalian species. The release is not the result of antigen-antibody dissociation or extensive degradation of the complexes. However, the released complexes have been altered because they will no longer bind to fresh lymphocytes. The release is not the result of lymphocyte damage mediated by complement. It is complement-dependent, and is generated either preferentially or exclusively via the alternate pathway, since it occurs in C4-deficient serum, is Mg(++) but not Ca(++) dependent, and requires C3 proactivator. C3 inactivator is not involved. The release activity of the serum, once generated, is unstable at 37 degrees C. The release of complexes from the lymphocyte membrane by serum provides a convenient assay for the functioning of the alternate pathway in the mouse and in other species.

MeSH Terms
Animals Antigen-Antibody Complex Autoradiography B-Lymphocytes/immunology Calcium Cell Membrane/immunology Chromium Isotopes Complement System Proteins Humans Immune Sera Kinetics Magnesium Mice Mice, Inbred AKR Mice, Inbred CBA Serum Albumin, Radio-Iodinated Sheep/immunology Temperature
Chemicals
Antigen-Antibody Complex Chromium Isotopes Immune Sera Serum Albumin, Radio-Iodinated Complement System Proteins Magnesium Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller G W
Saluk P H
Nussenzweig V
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38 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.138.3.495
Published
1973-09-01
Pages
495-507
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139414
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.138.3.495 PMC Full Text View journal details
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