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PMID: 4543458 Published · ppublish English Journal Article

Specific murine B-cell activation by synthetic single-and double-stranded polynucleotides.

The Journal of experimental medicine ·Vol. 138 ·No. 6 ·1973-12-01 ·Pages 1545-63

Scher I, Strong DM, Ahmed A, Knudsen RC, Sell KW

Abstract

The synthetic single- and double-stranded polynucleotides, poly I, poly C, and poly I.C, were shown to induce thymidine incorporation in six inbred strains of murine spleen cells. This stimulation was shown to be secondary to B-cell activation and not due to contamination of the polynucleotides with bacterial lipopolysaccharide (LPS). The ability of poly I.C to act as a B-cell mitogen, in addition to its behavior as a thymic-independent antigen, suggested that these two phenomena may be related. The similarity of the molecular structure of poly I.C to LPS, a material which also acts as a thymic-independent antigen and a B-cell mitogen, supports the hypothesis that the polyvalent nature of these materials accounts for their functional interaction with murine B cells.

MeSH Terms
Animals Antigens B-Lymphocytes/growth & development,immunology,metabolism Cell Division Immune Sera Mice Mice, Inbred DBA Mitogens Poly I-C/pharmacology Spleen/cytology,immunology Thymectomy Thymidine/metabolism Thymus Gland/immunology Tritium
Chemicals
Antigens Immune Sera Mitogens Tritium Poly I-C Thymidine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Scher I
Strong D M
Ahmed A
Knudsen R C
Sell K W
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48 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.138.6.1545
Published
1973-12-01
Pages
1545-63
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139448
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.138.6.1545 PMC Full Text View journal details
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