Analysis of eucaryotic DNAs with a restriction endonuclease from H. influenzae: isolation of "hidden" satellite DNAs.

Nucleic acids research ·Vol. 1 ·No. 9 ·1974-09-00 ·Pages 1099-120

Roizés G

Abstract

The restriction enzymes from H.influenzae have been used to study various eucaryotic DNAs. Fractions resistant to the action of these enzymes have been isolated from rat, mouse and calf DNAs. The method revealed the existence in rat of several components having the properties of satellite DNAs. Mouse DNA was shown to contain a new satellite of buoyant density 1. 704. Therefore, this appears to be a powerfull method for the isolation of "hidden" satellite DNAs. Calf satellite DNAs rho = 1.705 and rho = 1.723 were those resistant to the action of the restriction enzymes whilst satellite DNAs rho = 1.714 and rho = 1.710 gave fragments of discrete lengths, suggesting internal repeat units of 1 500 and 5 000 base pairs respectively.

MeSH Terms

Animals Cattle Centrifugation, Density Gradient DNA Deoxyribonucleases Endonucleases Haemophilus influenzae/enzymology Liver/analysis Methods Molecular Weight Nucleic Acid Conformation Nucleic Acid Renaturation Rats Species Specificity Thymus Gland/analysis

Chemicals

DNA Deoxyribonucleases Endonucleases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Roizés G

References (13)

13 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/1.9.1099

Published

1974-09-00

Pages

1099-120

Language

English

Region

England

NLM ID

0411011

PMCID

PMC344334

Subset

External Links

PubMed source DOI: 10.1093/nar/1.9.1099 PMC Full Text View journal details

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Analysis of eucaryotic DNAs with a restriction endonuclease from H. influenzae: isolation of "hidden" satellite DNAs.

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