A mutant of Escherichia coli that requires high concentrations of biotin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 3 ·1972-03-00 ·Pages 676-80

Campbell A, Del Campillo-Campbell A, Chang R

Abstract

A recessive mutation (bir) of E. coli, at 77 min on the standard map, destroys ability of the bacteria to take up biotin or to grow except at high concentrations of biotin. In a biotin prototroph, the bir mutation causes overproduction and excretion of biotin, and derepression of at least one biosynthetic gene (bioD). Growth of the bir mutant is inhibited by avidin or high concentrations of dethiobiotin. Both effects are reversed by biotin.

MeSH Terms

Biotin/biosynthesis,metabolism Caproates Chromosome Mapping Coliphages Enzyme Repression Escherichia coli/enzymology,growth & development,metabolism Genes Genes, Dominant Genes, Recessive Genotype Heterozygote Imidazoles Ligases/analysis Mutation Recombination, Genetic Transduction, Genetic

Chemicals

Caproates Imidazoles Biotin Ligases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Campbell A

Del Campillo-Campbell A

Chang R

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.3.676

Published

1972-03-00

Pages

676-80

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC426533

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.3.676 PMC Full Text View journal details

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A mutant of Escherichia coli that requires high concentrations of biotin.

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