Large-scale production of lambda bacteriophage and purified lambda deoxyribonucleic acid.

Applied microbiology ·Vol. 23 ·No. 4 ·1972-04-00 ·Pages 791-5

De Czekala A, Luk D, Bartl P

Abstract

Large amounts of a heat-inducible phage lambda mutant (lambdaCI857) may be obtained under standardized conditions. The phage is harvested by simple polyethylene glycol (C 6000) precipitation and purified by CsCl density gradient banding. Deoxyribonucleic acid (DNA) is extracted by cold phenol and purified by sucrose density gradient sedimentation to yield a homogenous population of unbroken lambda DNA molecules.

MeSH Terms

Cell Count Centrifugation, Density Gradient Cesium Chemical Precipitation Chlorides Coliphages/analysis,growth & development,isolation & purification Culture Media DNA, Viral/isolation & purification Escherichia coli/growth & development Glycols Hot Temperature Lysogeny Methods Mutation Phenols Solvents Sucrose Virus Cultivation

Chemicals

Chlorides Culture Media DNA, Viral Glycols Phenols Solvents Cesium Sucrose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

De Czekala A

Luk D

Bartl P

References (6)

6 references, click to expand

On the mechanism of transcription of the lambda genome during induction of lysogenic bacteria.
J Mol Biol. 1967 May 14;25(3):517-36 PMID: 6035288
[On a thermosensitive repression system in the Escherichia coli lambda bacteriophage].
C R Hebd Seances Acad Sci. 1962 Feb 19;254:1517-9 PMID: 13918507
[On the nature of the repressor ensuring the immunity of lysogenic bacteria].
C R Hebd Seances Acad Sci. 1962 Jun 13;254:4214-6 PMID: 14036499
ULTRAVIOLET SENSITIVITY OF ESCHERICHIA COLI CONTAINING HEAT-INDUCIBLE LAMBDA PROPHAGES.
Science. 1964 Jul 10;145(3628):175-6 PMID: 14171560
Acetylornithinase of Escherichia coli: partial purification and some properties.
J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
[Research on the lysogenic Bacillus megatherium].
Ann Inst Pasteur (Paris). 1950 Jun;78(6):711-39 PMID: 15425966

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.23.4.791-795.1972

Published

1972-04-00

Pages

791-5

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380437

Subset

External Links

PubMed source DOI: 10.1128/am.23.4.791-795.1972 PMC Full Text

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Large-scale production of lambda bacteriophage and purified lambda deoxyribonucleic acid.

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