Isolation of morphological mutants of Agrobacterium tumefaciens.

Journal of bacteriology ·Vol. 110 ·No. 2 ·1972-05-00 ·Pages 743-6

Fujiwara T, Fukui S

Abstract

Morphological mutants were isolated from a wild strain of Agrobacterium tumefaciens at a high frequency by treatment with a nitrosoguanidine. Seventeen of the 20 mutants isolated were temperature-sensitive. At 27 C, the mutant cells were rod-shaped and at 37 C, spherical or branched, whereas the wild-type cells were rod-shaped at both temperatures.

MeSH Terms

Culture Media Genetics, Microbial Micropore Filters Microscopy, Phase-Contrast Mutagens/pharmacology Mutation Nitrosoguanidines/pharmacology Rhizobium/cytology,drug effects,growth & development,isolation & purification Temperature

Chemicals

Culture Media Mutagens Nitrosoguanidines

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fujiwara T

Fukui S

References (9)

9 references, click to expand

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Proc Natl Acad Sci U S A. 1966 Oct;56(4):1290-7 PMID: 4381935
Purification and properties of 3-ketosucrose-forming enzyme from the cells of Agrobacterium tumefaciens.
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Phosphoglucomutase mutants of Neurospora sitophila and their relation to morphology.
Proc Natl Acad Sci U S A. 1970 Jul;66(3):638-45 PMID: 5269231
Induction of pleomorphy and calcium ion deficiency in Lactobacillus bifidus.
J Bacteriol. 1970 Apr;102(1):217-20 PMID: 5437726
Cell wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology.
Nature. 1968 Jul 20;219(5151):285-8 PMID: 4970364
Regulation of Cell Division in Escherichia coli: Characterization of Temperature-Sensitive Division Mutants.
J Bacteriol. 1970 Dec;104(3):1052-64 PMID: 16559077
A LOCUS THAT CONTROLS FILAMENT FORMATION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI K-12.
Genetics. 1964 Feb;49:237-46 PMID: 14124942
Phosphoglucomutase mutants and morphological changes in neurospora crassa.
Proc Natl Acad Sci U S A. 1967 Sep;58(3):923-30 PMID: 5233848
ANALYSIS OF A GENE CONTROLLING CELL DIVISION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI.
J Bacteriol. 1964 Mar;87:720-6 PMID: 14127589

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.110.2.743-746.1972

Published

1972-05-00

Pages

743-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247472

Subset

External Links

PubMed source DOI: 10.1128/jb.110.2.743-746.1972 PMC Full Text View journal details

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Isolation of morphological mutants of Agrobacterium tumefaciens.

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