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PMID: 4555405 Published · ppublish English Journal Article

New amino acid regulatory locus having unusual properties in heterozygous merodiploids.

Journal of bacteriology ·Vol. 110 ·No. 3 ·1972-06-00 ·Pages 1127-34

Kline EL

Abstract

Spontaneous mutants of Escherichia coli B/r resistant to 5',5',5',-trifluoro-dl-leucine contain defects in a gene which maps to the left of the threonine region. Low-level constitutive expression of the isoleucine-valine and leucine operons is caused by this mutation in haploid strains. This is in contrast to extremely high levels of gene expression in the heterozygous merodiploids (F' wild type/mutant allele). The properties of these mutants define a new locus and suggest that it encodes a subunit protein which is involved in the repression of the structural genes for the branched-chain amino acid pathways.

MeSH Terms
Acyltransferases/metabolism Alleles Amino Acids/biosynthesis Cell-Free System Chromosome Mapping Chromosomes, Bacterial Culture Media Dicarboxylic Acids Diploidy Drug Resistance, Microbial Escherichia coli/drug effects,enzymology,growth & development,metabolism Fluorine/pharmacology Genes Heterozygote Hydro-Lyases/metabolism Leucine/pharmacology Lyases/metabolism Mutation Pyruvates Recombination, Genetic Threonine Transduction, Genetic Valerates
Chemicals
Amino Acids Culture Media Dicarboxylic Acids Pyruvates Valerates Fluorine Threonine Acyltransferases Lyases Hydro-Lyases Leucine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kline E L
References (38)
38 references, click to expand
  1. Expression of the leucine operon.
    J Bacteriol. 1966 Apr;91(4):1570-6 PMID: 5326118
  2. A regulator gene that is dominant on an episome and recessive on a chromosome.
    Proc Natl Acad Sci U S A. 1965 Oct;54(4):1084-91 PMID: 5327253
  3. Regulation of the enzymes of the tryptophan pathway in Escherichia coli.
    Genetics. 1965 Dec;52(6):1303-16 PMID: 5327408
  4. Orientation of the isoleucine-valine genes in the Salmonella typhimurium linkage map.
    Genetics. 1966 May;53(5):971-6 PMID: 5333374
  5. Histidine regulatory mutants in Salmonella typhimurium II. Histidine regulatory mutants having altered histidyl-tRNA synthetase.
    J Mol Biol. 1966 Dec 28;22(2):325-33 PMID: 5339688
  6. Further evidence for positive control of the L-arabinose system by gene araC.
    J Mol Biol. 1967 May 14;25(3):443-54 PMID: 5340694
  7. Roles of amino acid activating enzymes in cellular physiology.
    Bacteriol Rev. 1966 Dec;30(4):701-19 PMID: 5342516
  8. Mutants of Escherichia coli with an altered tryptophanyl-transfer ribonucleic acid synthetase.
    J Bacteriol. 1968 Apr;95(4):1283-94 PMID: 4869215
  9. Isoleucine and valine metabolism of Escherichia coli. XV. Biochemical properties of mutants resistant to thiaisoleucine.
    J Bacteriol. 1968 May;95(5):1672-9 PMID: 4297021
  10. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.
    Proc Natl Acad Sci U S A. 1968 May;60(1):160-7 PMID: 4873517
  11. Regulation of branched-chain amino acid biosynthesis in Salmonella typhimurium: isolation of regulatory mutants.
    J Bacteriol. 1969 Mar;97(3):1272-82 PMID: 4887507
  12. Metabolic interlock. Regulatory interactions exerted between biochemical pathways.
    J Biol Chem. 1969 Jun 10;244(11):2816-23 PMID: 4306282
  13. Arabinose-leucine deletion mutants of Escherichia coli B-r.
    J Bacteriol. 1969 Jun;98(3):1159-69 PMID: 4892369
  14. Antipolarity in the ilv operon of Escherichia coli K-12.
    J Bacteriol. 1969 Jun;98(3):1179-94 PMID: 4892370
  15. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  16. Amber mutants of the trpR regulatory gene.
    J Mol Biol. 1969 Aug 28;44(1):185-93 PMID: 4897801
  17. Operator constitutive mutations in the leucine operon of Salmonella typhimurium.
    Genetics. 1969 Apr;61(4):777-87 PMID: 4903803
  18. Metabolic interlock. The influence of histidine on tryptophan biosynthesis in Bacillus subtilis.
    J Biol Chem. 1970 May 10;245(9):2384-90 PMID: 4315152
  19. Tryptophanyl transfer RNA synthetase and expression of the tryptophan operon in the trpS mutants of Escherichia coli.
    Genetics. 1969 Mar;61(3):521-38 PMID: 4914847
  20. Current linkage map of Escherichia coli.
    Bacteriol Rev. 1970 Jun;34(2):155-75 PMID: 4918631
  21. Specific binding of leucyl transfer RNA to an immature form of L-threonine deaminase: its implications in repression.
    Proc Natl Acad Sci U S A. 1970 Aug;66(4):1027-35 PMID: 4920088
  22. Evidence that tryptophanyl transfer ribonucleic acid is not the corepressor of the tryptophan operon of Escherichia coli.
    J Bacteriol. 1971 Jan;105(1):268-75 PMID: 5541012
  23. Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase.
    J Bacteriol. 1971 Feb;105(2):527-37 PMID: 5541530
  24. Mutants of Salmonella typhimurium with an altered leucyl-transfer ribonucleic acid synthetase.
    J Bacteriol. 1971 Apr;106(1):213-20 PMID: 4928008
  25. Regulatory gene mutations affecting arginine biosynthesis in Escherichia coli.
    Mol Gen Genet. 1971;111(1):1-14 PMID: 4932243
  26. Genetic mapping of the regulator and operator genes of the lac operon.
    J Mol Biol. 1968 Sep 28;36(3):413-7 PMID: 4939632
  27. Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.
    J Bacteriol. 1971 Nov;108(2):742-50 PMID: 4942762
  28. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  29. Genetic control of induction of alkaline phosphatase synthesis in E. coli.
    Proc Natl Acad Sci U S A. 1962 Aug;48:1398-402 PMID: 13896931
  30. Genetic fine structure of the leucine operon in Salmonella.
    Genetics. 1963 Mar;48:441-57 PMID: 13933019
  31. Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression.
    Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804-8 PMID: 13959618
  32. ON THE INCORPORATION OF 5',5',5'-TRIFLUOROLEUCINE INTO PROTEINS OF E. COLI.
    Biochemistry. 1963 May-Jun;2:471-6 PMID: 14069531
  33. ISOLEUCINE-VALINE REQUIRING MUTANTS OF SALMONELLA TYPHIMURIUM.
    Genetics. 1964 Nov;50:957-65 PMID: 14239778
  34. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES.
    J Bacteriol. 1965 Mar;89:654-60 PMID: 14273640
  35. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES.
    J Bacteriol. 1965 Mar;89:661-4 PMID: 14273641
  36. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. I. GENETIC DEREPRESSION OF ENZYME FORMATION.
    J Bacteriol. 1964 Mar;87:566-73 PMID: 14127571
  37. ROLE OF VALYL-SRNA SYNTHETASE IN ENZYME REPRESSION.
    Proc Natl Acad Sci U S A. 1965 Mar;53:539-43 PMID: 14338232
  38. Determination of the order of mutational sites governing L-arabinose utilization in Escherichia coli B/r bv transduction with phage Plbt.
    Virology. 1959 Nov;9:314-31 PMID: 13829634
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.110.3.1127-1134.1972
Published
1972-06-00
Pages
1127-34
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247536
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.110.3.1127-1134.1972 PMC Full Text View journal details
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