Mechanism of the inactivation of the bacteriophage T 1 in aerosols.

Applied microbiology ·Vol. 23 ·No. 5 ·1972-05-00 ·Pages 938-41

Trouwborst T, de Jong JC

Abstract

The mechanism of inactivation of bacteriophage T(1) in aerosols was studied by using (32)P-labeled phage. During inactivation, viability decreased in parallel with the adsorption of (32)P to the host, showing either that inactivated phage does not adsorb or that the deoxyribonucleic acid (DNA) has left the coat. A (32)P band at the density of free DNA was found when inactivated phage was analyzed in a CsCl gradient.

MeSH Terms

Adsorption Aerosols Air Microbiology Centrifugation, Density Gradient Cesium Chlorides Coliphages/analysis,isolation & purification DNA, Viral/analysis Escherichia coli Humidity Lysogeny Phosphorus Isotopes Viral Proteins/analysis

Chemicals

Aerosols Chlorides DNA, Viral Phosphorus Isotopes Viral Proteins Cesium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Trouwborst T

de Jong J C

References (8)

8 references, click to expand

Multistage liquid impinger.
Bacteriol Rev. 1966 Sep;30(3):559-70 PMID: 5917336
Isolation and physicochemical investigation of T1 bacteriophage DNA.
Virology. 1967 Sep;33(1):1-9 PMID: 6039960
The pathogenicity in mice of aerosols of encephalomyocarditis group viruses or their infectious nucleic acids.
J Immunol. 1968 Jan;100(1):120-7 PMID: 4295061
Survival of a picornavirus and its infectious ribonucleic acid after aerosolization.
Appl Microbiol. 1968 Nov;16(11):1811-3 PMID: 4302283
The inactivation of poliovirus in aerosols.
J Hyg (Lond). 1968 Dec;66(4):557-65 PMID: 4304384
Rapid equilibrium isopycnic CsC1 gradients.
Biochim Biophys Acta. 1969 Mar 18;179(1):136-44 PMID: 4894516
Biological activity of the nucleic acids extracted from two aerosolized bacterial viruses.
Appl Microbiol. 1971 Apr;21(4):761-2 PMID: 4995853
The efficiency of various liquid impinger samplers in bacterial aerosols.
Br J Ind Med. 1957 Oct;14(4):287-97 PMID: 13471876

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.23.5.938-941.1972

Published

1972-05-00

Pages

938-41

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380474

Subset

External Links

PubMed source DOI: 10.1128/am.23.5.938-941.1972 PMC Full Text

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Mechanism of the inactivation of the bacteriophage T 1 in aerosols.

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