DNA binding activity of serum from patients with systemic lupus erythematosus.

Clinical and experimental immunology ·Vol. 35 ·No. 3 ·1979-03-00 ·Pages 356-63

Halsey JF, Woolery WA, Oleinick S, Kahaleh MB, Leroy EC

Abstract

The reaction conditions essential for reproducible use of the cellulose ester membrane filter assay for anti-DNA antibody levels in patients with systemic lupus erythematosus are described. A dependence of DNA-binding capacity on serum concentration was observed in the assay, requiring that serum concentrations be comparable in determinations of DNA-binding activity of different sera and when comparing different published studies. The DNA-binding capacity of serum from lupus patients was found to be significantly different from that of healthy controls. However, the binding capacities were not significantly different between lupus patients with and without nephritis. The relative avidity of the anti-DNA antibodies were estimated from plots of 1/DNA bound vs 1/DNA free and these data indicate that the avidities of the antibodies from the two groups of lupus patients are not significantly different. This observation suggests that the tightness of binding between the DNA and the serum anti-DNA antibodies cannot be used to predict immune complex-induced nephritis in lupus patients.

MeSH Terms

Antibodies/analysis,immunology Antibody Affinity Blood Volume DNA/immunology Female Humans Lupus Erythematosus, Systemic/complications,immunology Male Molecular Weight Nephritis/complications,immunology

Chemicals

Antibodies DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Halsey J F

Woolery W A

Oleinick S

Kahaleh M B

Leroy E C

References (25)

25 references, click to expand

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Article Info

Journal

Clinical and experimental immunology

Abbr.

Clin Exp Immunol

ISSN

0009-9104

Published

1979-03-00

Pages

356-63

Language

English

Region

England

NLM ID

0057202

PMCID

PMC1537628

Subset

External Links

PubMed source PMC Full Text View journal details

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DNA binding activity of serum from patients with systemic lupus erythematosus.

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