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PMID: 4559592 Published · ppublish English Journal Article

The stabilization of episomal integration by genetic inversion: a general hypothesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 8 ·1972-08-00 ·Pages 2061-5

Adelberg EA, Bergquist P

Abstract

In F' cells of Escherichia coli K12, recombination in the region of genetic duplication between chromosome and F-genote produces a rapid alteration between the integrated and detached states of the episome. Nevertheless, F' strains can give rise to Hfr cells in which the F-genote has undergone stable integration. We have found that one such Hfr has retained the genetic duplication as well as the recombination proficiency of the parental F' strain; to account for the failure of recombination to detach the episome in this Hfr, we have postulated the inversion of one of the duplicated regions. A general model is presented, showing that an inversion must prevent episome detachment by any combination of genetic crossovers.

MeSH Terms
Chromosome Aberrations Chromosomes, Bacterial Coliphages Conjugation, Genetic Crossing Over, Genetic Escherichia coli/enzymology,metabolism,radiation effects Extrachromosomal Inheritance Fucose/metabolism Galactose/metabolism Glucose Isomerases/biosynthesis Lysogeny Models, Biological Radiation Effects Recombination, Genetic Ultraviolet Rays
Chemicals
Fucose Isomerases Glucose Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Adelberg E A
Bergquist P
References (10)
10 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.8.2061
Published
1972-08-00
Pages
2061-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426868
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.8.2061 PMC Full Text View journal details
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