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PMID: 4559728 Published · ppublish English Journal Article

R factor elimination during thymine starvation: effects of inhibition of protein synthesis and readdition of thymine.

Journal of bacteriology ·Vol. 111 ·No. 2 ·1972-08-00 ·Pages 361-7

Pinney RJ, Smith JT

Abstract

R factor 1818 is shown to be eliminated from a thymineless strain of Escherichia coli J6-2 (R-1818) during thymine starvation. Readdition of thymine to the starved cultures produces a partial recovery in viable count but does not affect the proportion of R(-) cells. The R factor is not cured from exponential- or stationary-phase cultures which are starved of required amino acids as well as thymine, nor from cells which are deprived of thymine in the presence of chloramphenicol. However, in both of these cases, the extent of thymineless death is reduced. It is suggested that protein synthesis is a requirement for R-1818 elimination, and the possible nature of this protein is discussed.

MeSH Terms
Amino Acids/metabolism Bacterial Proteins/biosynthesis Bacteriological Techniques Cell Survival Chloramphenicol/pharmacology Culture Media Drug Resistance, Microbial Escherichia coli/drug effects,growth & development,metabolism Extrachromosomal Inheritance Genetics, Microbial Mutation Streptomycin/pharmacology Tetracycline/pharmacology Thymine/metabolism
Chemicals
Amino Acids Bacterial Proteins Culture Media Chloramphenicol Tetracycline Thymine Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pinney R J
Smith J T
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29 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.111.2.361-367.1972
Published
1972-08-00
Pages
361-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251291
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.111.2.361-367.1972 PMC Full Text View journal details
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