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PMID: 4562740 Published · ppublish English Journal Article

Initiation factor 2-dependent ribosomal binding of N-formylmethionyl-transfer RNA without added guanosine triphosphate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 10 ·1972-10-00 ·Pages 2770-3

Mazumder R

Abstract

Evidence is presented that suggests that GTP (or 5'-guanylylmethylene diphosphonate) is not essential for either formation of an AUG-directed, initiation factor IF-2-dependent initiation complex on the 30S subunit or for positioning of fMet-tRNA on the peptidyl site. However, recycling of limiting amounts of IF-2 requires both GTP and the 50S subunit. The formation of the complex IF-2-30S as an intermediate in polypeptidechain initiation is suggested.

MeSH Terms
Binding Sites Carbon Isotopes Escherichia coli/metabolism Formates/metabolism Guanosine Triphosphate/pharmacology Methionine/metabolism Osmolar Concentration Peptide Chain Initiation, Translational/drug effects Peptide Initiation Factors/metabolism Protein Binding/drug effects Puromycin/pharmacology RNA, Bacterial/metabolism RNA, Transfer/metabolism Ribosomes/drug effects,metabolism Stimulation, Chemical
Chemicals
Carbon Isotopes Formates Peptide Initiation Factors RNA, Bacterial Puromycin Guanosine Triphosphate RNA, Transfer Methionine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Mazumder R
References (8)
8 references, click to expand
  1. Recognition of bacterial initiator tRNA by an initiation factor.
    Nat New Biol. 1971 May 19;231(20):76-8 PMID: 4931882
  2. Polypeptide chain initiation in eukaryotes: functional identity of supernatant factor from various sources.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1796-9 PMID: 4558658
  3. Entry site of formylmethionyl-tRNA.
    FEBS Lett. 1972 Feb 15;20(3):347-351 PMID: 11946455
  4. Peptide chain elongation.
    Cold Spring Harb Symp Quant Biol. 1969;34:437-54 PMID: 4314910
  5. Requirement for GTP in the initiation process on reticulocyte ribosomes and ribosomal subunits.
    Proc Natl Acad Sci U S A. 1971 Sep;68(9):2246-51 PMID: 5289383
  6. A supernatant factor involved in initiation complex formation with eukaryotic ribosomes.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3059-63 PMID: 4943553
  7. Studies on polypeptide chain initiation factors F(1) and F(2).
    FEBS Lett. 1971 Oct 15;18(1):64-66 PMID: 11946083
  8. A complex between initiation factor IF2, guanosine triphosphate, and fMet-tRNA: an intermediate in initiation complex formation.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3122-6 PMID: 4943554
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.10.2770
Published
1972-10-00
Pages
2770-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389641
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.10.2770 PMC Full Text View journal details
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