The threonine-sensitive homoserine dehydrogenase and aspartokinase activities of Escherichia coli K12. The two catalytic activities are carried by two independent regions of the polypeptide chain.

Alcohol Oxidoreductases Allosteric Regulation Amino Acids/analysis Aspartic Acid Binding Sites Centrifugation, Density Gradient Electrophoresis, Disc Escherichia coli/enzymology Homoserine Macromolecular Substances Molecular Weight Mutation Peptide Hydrolases Phosphotransferases/isolation & purification Protein Conformation Sodium Dodecyl Sulfate Threonine

Amino Acids Macromolecular Substances Threonine Aspartic Acid Sodium Dodecyl Sulfate Homoserine Alcohol Oxidoreductases Phosphotransferases Peptide Hydrolases