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PMID: 4563594 Published · ppublish English Journal Article

Effect of bacteriophage ghost infection on protein synthesis in Escherichia coli.

Journal of virology ·Vol. 10 ·No. 4 ·1972-10-00 ·Pages 713-20

Fukuma I, Kaji A

Abstract

The rate of protein synthesis by Escherichia coli markedly decreased within 1 min after phage T4 infection, whereas a complete cessation of protein synthesis was observed within at least 25 sec after T4 ghost infection. The cellular level of amino acids and aminoacyl-transfer ribonucleic acid (tRNA) did not change drastically upon infection with ghosts, indicating that the inhibition of protein synthesis took place at a step(s) beyond aminoacyl-tRNA formation. The host messenger RNA remained intact and still bound to ribosomes shortly after ghost infection. Kinetic studies of the effect of ghosts on host protein synthesis revealed that nascent peptide chains on ribosomes were not released upon ghost infection.

MeSH Terms
Amino Acids/metabolism Bacterial Proteins/antagonists & inhibitors,biosynthesis Carbon Isotopes Coliphages Escherichia coli/enzymology,metabolism Galactosidases/biosynthesis Kinetics Peptides/metabolism Protein Binding RNA, Bacterial/biosynthesis RNA, Messenger/metabolism RNA, Transfer/metabolism Ribosomes/metabolism Time Factors Uracil/metabolism Valine/metabolism Viral Proteins/isolation & purification,pharmacology
Chemicals
Amino Acids Bacterial Proteins Carbon Isotopes Peptides RNA, Bacterial RNA, Messenger Viral Proteins Uracil RNA, Transfer Galactosidases Valine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fukuma I
Kaji A
References (28)
28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.10.4.713-720.1972
Published
1972-10-00
Pages
713-20
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC356525
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.10.4.713-720.1972 PMC Full Text View journal details
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