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PMID: 4566455 Published · ppublish English Journal Article

Purification and properties of D protein: a transcription factor of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 12 ·1972-12-00 ·Pages 3660-4

Ghosh S, Echols H

Abstract

D factor is protein that stimulates DNA transcription in vitro by RNA polymerase. For DNA from bacteriophage lambda, we present evidence that D protein interacts with DNA to increase the frequency and improve the specificity of initiation by RNA polymerase.

MeSH Terms
Bacterial Proteins/isolation & purification,metabolism DNA/metabolism DNA, Bacterial/metabolism DNA, Viral/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/analysis,metabolism Hot Temperature Nucleic Acid Denaturation Nucleic Acid Hybridization Phosphorus Isotopes Protein Binding RNA, Bacterial/analysis,biosynthesis,metabolism Templates, Genetic Thymus Gland Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Viral Phosphorus Isotopes RNA, Bacterial DNA DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ghosh S
Echols H
References (25)
25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.12.3660
Published
1972-12-00
Pages
3660-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389843
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.12.3660 PMC Full Text View journal details
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