Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 12 ·1972-12-00 ·Pages 3756-60

Rick PD, Osborn MJ

Abstract

A new type of auxotrophic mutant of Salmonella typhimurium has been isolated that is defective in the synthesis of the 3-deoxy-D-mannooctulosonate (ketodeoxyoctonate) region of the lipopolysaccharide and requires D-arabinose-5-phosphate for growth. Genetic and biochemical evidence indicated that the mutant defect was due to an altered ketodeoxyoctonate-8-phosphate synthetase with an apparent K(m) for D-arabinose-5-phosphate 35-fold higher than that of the parental enzyme. As a result of this enzymatic lesion, the mutant strain was dependent on exogenous D-arabinose-5-phosphate both for growth and for synthesis of a complete lipopolysaccharide.

MeSH Terms

Arabinose/metabolism Carbon Isotopes Galactose/metabolism Ligases/metabolism Lipopolysaccharides/biosynthesis Mutation Pentosephosphates Phosphates/metabolism Phosphoenolpyruvate Polysaccharides, Bacterial/biosynthesis Salmonella typhimurium/enzymology,isolation & purification,metabolism Sugar Acids/biosynthesis

Chemicals

Carbon Isotopes Lipopolysaccharides Pentosephosphates Phosphates Polysaccharides, Bacterial Sugar Acids 2-keto-3-deoxyoctonate Phosphoenolpyruvate Arabinose Ligases Galactose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Rick P D

Osborn M J

References (23)

23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.12.3756

Published

1972-12-00

Pages

3756-60

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389865

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.12.3756 PMC Full Text View journal details

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Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate).

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